Literature DB >> 3487396

Isolation and characterisation of lactoferrin separated from human whey by adsorption chromatography using Cibacron Blue F3G-A linked affinity adsorbent.

W R Bezwoda, N Mansoor.   

Abstract

A new one-step procedure for isolating lactoferrin from human whey using Cibacron Blue affinity chromatography is described. Characteristics of the isolated lactoferrin include pI 5.5-6.1 and differential migration on SDS-polyacrylamide gel electrophoresis of apo and iron saturated lactoferrin. The concentration of lactoferrin in breast milk varies with time after parturition but the iron saturation remains relatively constant with time.

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Year:  1986        PMID： 3487396     DOI： 10.1016/0009-8981(86)90321-9

Source DB:  PubMed          Journal:  Clin Chim Acta        ISSN： 0009-8981            Impact factor:   3.786


  2 in total

1.  On the molecular interaction between lactoferrin and the dye Red HE-3b. A novel approach for docking a charged and highly flexible molecule to protein surfaces.

Authors:  Mariano Grasselli; Osvaldo Cascone; F Birger Anspach; Jose M Delfino
Journal:  J Comput Aided Mol Des       Date:  2002-12       Impact factor: 3.686

2.  Multiple molecular forms of human lactoferrin. Identification of a class of lactoferrins that possess ribonuclease activity and lack iron-binding capacity.

Authors:  P Furmanski; Z P Li; M B Fortuna; C V Swamy; M R Das
Journal:  J Exp Med       Date:  1989-08-01       Impact factor: 14.307
  2 in total

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