Literature DB >> 3486920

A rapid ELISA for measurement of antibodies to nucleic acid antigens using UV-treated polystyrene microplates.

M Zouali, B D Stollar.   

Abstract

Pretreatment of polystyrene microplate wells with certain doses of UV light enhances their capacity for binding to single-stranded DNA, double stranded DNA and various synthetic polynucleotides. The use of UV-irradiated plates to immobilize nucleic acid antigens provides a simple, rapid, and specific ELISA for measuring anti-nucleic acid antibodies. The assay is at least as sensitive as the more complex method of precoating plates with poly(L-lysine). It is useful for detection of anti-DNA antibodies in sera of systemic lupus erythematous patients, as well as in culture fluids of murine and human anti-DNA-secreting hybridomas.

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Year:  1986        PMID： 3486920     DOI： 10.1016/0022-1759(86)90390-x

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  12 in total

1.  Immunochemical detection of multiple conformations within a 36 base pair oligonucleotide.

Authors:  D G Sanford; K J Kotkow; B D Stollar
Journal:  Nucleic Acids Res       Date:  1988-11-25       Impact factor: 16.971

2.  Antibody-nucleic acid interactions. Antibodies to psoralen-modified RNA as probes of RNA structure.

Authors:  D J Hurt; K D Winestock; M L O'Connor; M I Johnston
Journal:  Nucleic Acids Res       Date:  1987-11-11       Impact factor: 16.971

3.  Disease activity in systemic lupus erythematosus related to a range of antibodies binding DNA and synthetic polynucleotides.

Authors:  D A Isenberg; C Dudeney; W Williams; A Todd-Pokropek; B D Stollar
Journal:  Ann Rheum Dis       Date:  1988-09       Impact factor: 19.103

4.  Somatic diversification in the heavy chain variable region genes expressed by human autoantibodies bearing a lupus-associated nephritogenic anti-DNA idiotype.

Authors:  C Demaison; P Chastagner; J Thèze; M Zouali
Journal:  Proc Natl Acad Sci U S A       Date:  1994-01-18       Impact factor: 11.205

5.  Expansion of immunoglobulin-secreting cells and defects in B cell tolerance in Rag-dependent immunodeficiency.

Authors:  Jolan E Walter; Francesca Rucci; Laura Patrizi; Mike Recher; Stephan Regenass; Tiziana Paganini; Marton Keszei; Itai Pessach; Philipp A Lang; Pietro Luigi Poliani; Silvia Giliani; Waleed Al-Herz; Morton J Cowan; Jennifer M Puck; Jack Bleesing; Tim Niehues; Catharina Schuetz; Harry Malech; Suk See DeRavin; Fabio Facchetti; Andrew R Gennery; Emma Andersson; Naynesh R Kamani; JoAnn Sekiguchi; Hamid M Alenezi; Javier Chinen; Ghassan Dbaibo; Gehad ElGhazali; Adriano Fontana; Srdjan Pasic; Cynthia Detre; Cox Terhorst; Frederick W Alt; Luigi D Notarangelo
Journal:  J Exp Med       Date:  2010-06-14       Impact factor: 14.307

6.  Dipeptidyl peptidase IV (DP IV) activity in serum and on lymphocytes of MRL/Mp-lpr/lpr mice correlates with disease onset.

Authors:  T Kubota; H Iizuka; W W Bachovchin; B D Stollar
Journal:  Clin Exp Immunol       Date:  1994-05       Impact factor: 4.330

7.  Broad-spectrum antibodies against self-antigens and cytokines in RAG deficiency.

Authors:  Jolan E Walter; Lindsey B Rosen; Krisztian Csomos; Jacob M Rosenberg; Divij Mathew; Marton Keszei; Boglarka Ujhazi; Karin Chen; Yu Nee Lee; Irit Tirosh; Kerry Dobbs; Waleed Al-Herz; Morton J Cowan; Jennifer Puck; Jack J Bleesing; Michael S Grimley; Harry Malech; Suk See De Ravin; Andrew R Gennery; Roshini S Abraham; Avni Y Joshi; Thomas G Boyce; Manish J Butte; Kari C Nadeau; Imelda Balboni; Kathleen E Sullivan; Javeed Akhter; Mehdi Adeli; Reem A El-Feky; Dalia H El-Ghoneimy; Ghassan Dbaibo; Rima Wakim; Chiara Azzari; Paolo Palma; Caterina Cancrini; Kelly Capuder; Antonio Condino-Neto; Beatriz T Costa-Carvalho; Joao Bosco Oliveira; Chaim Roifman; David Buchbinder; Attila Kumanovics; Jose Luis Franco; Tim Niehues; Catharina Schuetz; Taco Kuijpers; Christina Yee; Janet Chou; Michel J Masaad; Raif Geha; Gulbu Uzel; Rebecca Gelman; Steven M Holland; Mike Recher; Paul J Utz; Sarah K Browne; Luigi D Notarangelo
Journal:  J Clin Invest       Date:  2015-10-12       Impact factor: 14.808

8.  Lupus anti-DNA antibodies bearing the 8.12 idiotype appear to be somatically mutated.

Authors:  A Livneh; E Behar; A Many; M Ehrenfeld; E Gazit; B Diamond
Journal:  J Clin Immunol       Date:  1992-01       Impact factor: 8.317

9.  Combined effects of FK506 (tacrolimus) and cyclophosphamide on atypical B220+ T cells, cytokine gene expression and disease activity in MRL/MpJ-lpr/lpr mice.

Authors:  J Woo; T M Wright; B Lemster; D Borochovitz; M A Nalesnik; A W Thomson
Journal:  Clin Exp Immunol       Date:  1995-04       Impact factor: 4.330

10.  In vivo treatment of (NZB X NZW)F1 lupus-like nephritis with monoclonal antibody to gamma interferon.

Authors:  C O Jacob; P H van der Meide; H O McDevitt
Journal:  J Exp Med       Date:  1987-09-01       Impact factor: 14.307
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