Literature DB >> 3486633

A simple and rapid chemical method for the determination of cephalosporins.

C Lübbe, Y Q Shen, A L Demain.   

Abstract

A simple and rapid chemical assay for cephalosporins is described. It is a simple modification of the colorimetric determination of penicillins in which the narrow spectrum beta-lactamase (penicillinase) is replaced by a broad spectrum beta-lactamase (cephalosporinase) produced by Enterobacter cloacae. The method can be used for assay of fermentation broths as well as for pure cephalosporins.

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Year:  1986        PMID： 3486633     DOI： 10.1007/BF02798577

Source DB:  PubMed          Journal:  Appl Biochem Biotechnol        ISSN： 0273-2289            Impact factor:   2.926


  2 in total

1.  Automated colorimetric determination of penicillin in fermentation samples using a molybdoarsenic acid-mercuric chloride reagent.

Authors:  K A Holm
Journal:  Anal Chem       Date:  1972-04-01       Impact factor: 6.986

2.  Beta-lactamase assays.

Authors:  G W Ross; C H O'Callaghan
Journal:  Methods Enzymol       Date:  1975       Impact factor: 1.600
  2 in total
  1 in total

1.  Comparison of the secondary metabolites in two scales of cephalosporin C (CPC) fermentation and two different post-treatment processes.

Authors:  Ying-Xiu Cao; Hua Lu; Bin Qiao; Yao Chen; Ying-Jin Yuan
Journal:  J Ind Microbiol Biotechnol       Date:  2012-10-09       Impact factor: 3.346
  1 in total

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