Literature DB >> 3485628

Effect of glycerol on Haemophilus influenzae transfection.

J H Stuy.   

Abstract

Competent Haemophilus influenzae bacteria were exposed to purified phage HP1 DNA and then plated for transfectants (PFU). When 32% (final concentration) glycerol was added before plating, between 10- and 100-fold more transfectants were observed. Glycerol had no significant effect on transfection with DNA from single or tandem double lysogens. It also had little effect on transformation with chromosomal DNA or on transformation of defective HP1 lysogens with phage HP1 DNA. It was concluded that glycerol induced the release of adsorbed linear double-stranded DNA into the interior of the cells.

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Year:  1986        PMID： 3485628      PMCID： PMC214589          DOI： 10.1128/jb.166.1.285-289.1986

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  29 in total

1.  INFECTION OF TRANSFORMABLE CELLS OF HAEMOPHILUS INFLUENZAE BY BACTERIOPHAGE AND BACTERIOPHAGE DNA.

Authors:  W HARM; C S RUPERT
Journal:  Z Vererbungsl       Date:  1963-12-30

2.  Prophage recombination in transformation -negative mutants of Haemophilus influenzae.

Authors:  J F Hoffmann; J H Stuy
Journal:  Biochem Biophys Res Commun       Date:  1972-02-16       Impact factor: 3.575

3.  Bacteriophage of Haemophilus influenzae. I. Differences between infection by whole phage, extracted phage DNA and prophage DNA extracted from lysogenic cells.

Authors:  M E Boling; J K Setlow; D P Allison
Journal:  J Mol Biol       Date:  1972-02-14       Impact factor: 5.469

4.  Prophage mapping by transformation.

Authors:  J H Stuy
Journal:  Virology       Date:  1969-08       Impact factor: 3.616

5.  Radiation-sensitive and radiation-resistant mutants of Haemophilus influenzae.

Authors:  B J Barnhart; S H Cox
Journal:  J Bacteriol       Date:  1968-07       Impact factor: 3.490

6.  Fate of transforming DNA in the Haemophilus influenzae transformation system.

Authors:  J H Stuy
Journal:  J Mol Biol       Date:  1965-09       Impact factor: 5.469

7.  Influence of transformability on the formation of superinfection double lysogens in Haemophilus influenzae.

Authors:  J H Stuy; J F Hoffmann
Journal:  J Virol       Date:  1971-01       Impact factor: 5.103

8.  Transformasomes: specialized membranous structures that protect DNA during Haemophilus transformation.

Authors:  M E Kahn; F Barany; H O Smith
Journal:  Proc Natl Acad Sci U S A       Date:  1983-11       Impact factor: 11.205

9.  Incomplete bacteriophage-like particles in ultraviolet-irradiated haemophilus.

Authors:  I Stachura; F W Mckinley; G Leidy; H E Alexander
Journal:  J Bacteriol       Date:  1969-05       Impact factor: 3.490

10.  Repair of deoxyribonucleic acid in Haemophilus influenzae. I. X-ray sensitivity of ultraviolet-sensitive mutants and their behavior as hosts to ultraviolet-irradiated bacteriophage and transforming deoxyribonucleic acid.

Authors:  J K Setlow; D C Brown; M E Boling; A Mattingly; M P Gordon
Journal:  J Bacteriol       Date:  1968-02       Impact factor: 3.490
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  2 in total

1.  sxy-1, a Haemophilus influenzae mutation causing greatly enhanced spontaneous competence.

Authors:  R J Redfield
Journal:  J Bacteriol       Date:  1991-09       Impact factor: 3.490

2.  rpe, a cis-acting element from the strA region of the Haemophilus influenzae chromosome that makes plasmid establishment independent of recombination.

Authors:  D McCarthy; S S Cox
Journal:  J Bacteriol       Date:  1986-10       Impact factor: 3.490
  2 in total

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