| Literature DB >> 34853973 |
S M Rodneva1, A A Osipov1,2, D V Guryev3,4, A A Tsishnatti1, Y А Fedotov1,2, E I Yashkina1, N Y Vorobyova1,2, A A Maksimov1, O A Kochetkov1, A S Samoylov1, A N Osipov1,2.
Abstract
We compared the formation of γH2AX foci (marker of DNA double-strand breaks) in human lung fibroblasts (MRC-5 line) during their 24-h incubation in a medium containing 3H-labeled thymidine or amino acids (glycine, alanine, and proline) with specific radioactivity from 100 to 400 MBq/liter. A linear dependence of changes in the number of γH2AX foci on the specific radioactivity of the medium was revealed. The quantitative yield of DNA double-strand breaks under the influence of 3H-thymidine was more than 2-fold higher than under the influence of 3H-labeled amino acids. Comparative analysis of the yields of DNA double-strand breaks during cell incubation with 3H-labeled amino acids showed that 3H-alanine produced more pronounced effect that 3H-proline, which is consistent with the data on the content of their non-radioactive analogs in chromatin proteins.Entities:
Keywords: 3H-amino acids; 3H-thymidine; MRC-5; tritium; γH2AX
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Year: 2021 PMID： 34853973 DOI： 10.1007/s10517-021-05370-6
Source DB: PubMed Journal: Bull Exp Biol Med ISSN： 0007-4888 Impact factor: 0.804