Molecular characterization of extended spectrum beta lactamases in clinical isolates of Escherichia coli and Klebsiella spp from a tertiary care hospital of South Eastern Assam.

Molecular characterization of ESBLs in clinical isolates of Escherichia coli and Klebsiella spp from a tertiary care hospital of South Eastern Assam was done by detection of blaTEM, blaSHV, blaCTX-M, blaOXA-2, blaOXA-10, blaPER, blaVEB and blaGES by Multiplex PCR. One hundred isolates yielded 44 bands of ESBL genes. CTX-M was most frequently isolated gene (36/44). Two isolates carried a combination of 2 genes CTX-M and OXA-2. Apart from CTX-M, the study isolates were also found to harbour TEM(n ​= ​3), OXA-2 (n ​= ​6) OXA-10 (n ​= ​1),GES(n ​= ​2) genes.