Literature DB >> 3485155

The importance of antibody quality in sandwich ELISA systems. Evaluation of selected commercial reagents.

J G Shields, M W Turner.   

Abstract

The detection of low levels of immunoglobulin in cell culture supernatants etc. by ELISA procedures demands high sensitivity and absolute specificity. We have used commercially available antibodies prepared by positive affinity purification and immunoglobulin fractions obtained by ion-exchange chromatography in various combinations in ELISA systems for such quantitative analyses. These demonstrate that when affinity-purified reagents lacking unwanted anti-species cross-reactivity are used as both the capture antibody and the indicator antibody the results are markedly superior (high sensitivity, low backgrounds and a wide working range) compared to those obtained with all other possible combinations.

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Year:  1986        PMID： 3485155     DOI： 10.1016/0022-1759(86)90340-6

Source DB:  PubMed          Journal:  J Immunol Methods        ISSN： 0022-1759            Impact factor:   2.303


  6 in total

1.  The role of opsonins in vacuolar sealing and the ingestion of zymosan by human neutrophils.

Authors:  A S Kemp; M W Turner
Journal:  Immunology       Date:  1986-09       Impact factor: 7.397

2.  Evaluation of C3b/C3bi opsonization and chemiluminescence with selected yeasts and bacteria using sera of different opsonic potential.

Authors:  M W Turner; C Grant; N D Seymour; B Harvey; R J Levinsky
Journal:  Immunology       Date:  1986-05       Impact factor: 7.397

3.  Correlation of immunoregulatory function with cell phenotype in cord blood lymphocytes.

Authors:  G Kingsley; C Pitzalis; A P Waugh; G S Panayi
Journal:  Clin Exp Immunol       Date:  1988-07       Impact factor: 4.330

4.  Agalactosyl IgG and beta-1,4-galactosyltransferase gene expression in rheumatoid arthritis patients and in the arthritis-prone MRL lpr/lpr mouse.

Authors:  P A Jeddi; K B Bodman-Smith; T Lund; P M Lydyard; L Mengle-Gaw; D A Isenberg; P Youinou; P J Delves
Journal:  Immunology       Date:  1996-04       Impact factor: 7.397

5.  Epstein-Barr-virus-transformed lymphoblastoid cell lines derived from patients with X-linked agammaglobulinaemia and Wiskott-Aldrich syndrome: responses to B cell growth and differentiation factors.

Authors:  Y L Lau; J G Shields; R J Levinsky; R E Callard
Journal:  Clin Exp Immunol       Date:  1989-02       Impact factor: 4.330

6.  Measurement of the concentration of murine IgG monoclonal antibody in hybridoma supernatants and ascites in absolute units by sensitive and reliable enzyme-linked immunosorbent assays (ELISA).

Authors:  J O Fleming; L B Pen
Journal:  J Immunol Methods       Date:  1988-05-25       Impact factor: 2.303
  6 in total

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