Xinhong Liu1,2,3,4, Xin Zuo5, Lijun Ma1,2,3,4, Qin Wang1,2,3,4, Lilan Zhu1,2,3,4, Li Li1,2,3,4, Xin Zhao2,3,4. 1. College of Biological and Chemical Engineering, Chongqing University of Education, Chongqing, People's Republic of China. 2. Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing, People's Republic of China. 3. Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing, People's Republic of China. 4. Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, People's Republic of China. 5. Department of Gastroenterology, The Sixth People's Hospital of Chongqing, Chongqing, People's Republic of China.
Abstract
BACKGROUND: Lung cancer has the characteristics of early metastasis, high recurrence, and high mortality rate despite emerging advances in diagnostic. Early diagnosis can significantly improve the patient's chances of cure and survival. PURPOSE: This study aimed to identify and assess a prognostic lncRNA/miRNA/gene signature in patients with lung cancer. METHODS: Pearson correlation analysis, univariate Cox analysis and LASSO Cox analysis were used to construct a lung cancer prognostic risk model based on m6A-related lncRNA. The interaction between lncRNA-miRNA-gene was verified by luciferase reporter gene experiment. RESULTS: The Pearson correlation analysis determined that 1655 lncRNAs significantly correlated with the expression of m6A genes. A lung cancer prognostic risk model, including 14 m6A-related lncRNAs, was constructed through univariate Cox analysis and least absolute shrinkage and selection operator (LASSO) Cox analysis. ABALON was identified as the key lncRNA through cluster analysis and gene expression difference analysis. CONCLUSION: It was experimentally verified that ABALON acted as a competing endogenous RNA by sponging miR-139-3p and indirectly regulated the expression of NOB1. This study provided a new biological target for the early diagnosis of lung cancer and a new direction for studying the mechanism of lung cancer.
BACKGROUND: Lung cancer has the characteristics of early metastasis, high recurrence, and high mortality rate despite emerging advances in diagnostic. Early diagnosis can significantly improve the patient's chances of cure and survival. PURPOSE: This study aimed to identify and assess a prognostic lncRNA/miRNA/gene signature in patients with lung cancer. METHODS: Pearson correlation analysis, univariate Cox analysis and LASSO Cox analysis were used to construct a lung cancer prognostic risk model based on m6A-related lncRNA. The interaction between lncRNA-miRNA-gene was verified by luciferase reporter gene experiment. RESULTS: The Pearson correlation analysis determined that 1655 lncRNAs significantly correlated with the expression of m6A genes. A lung cancer prognostic risk model, including 14 m6A-related lncRNAs, was constructed through univariate Cox analysis and least absolute shrinkage and selection operator (LASSO) Cox analysis. ABALON was identified as the key lncRNA through cluster analysis and gene expression difference analysis. CONCLUSION: It was experimentally verified that ABALON acted as a competing endogenous RNA by sponging miR-139-3p and indirectly regulated the expression of NOB1. This study provided a new biological target for the early diagnosis of lung cancer and a new direction for studying the mechanism of lung cancer.
Authors: Zeng Cheng Zou; Min Dai; Zeng Yin Huang; Yi Lu; He Ping Xie; Yi Fang Li; Yue Li; Ying Tan; Feng Lin Wang Journal: Oncol Res Date: 2018-02-08 Impact factor: 5.574