| Literature DB >> 34845607 |
David Witalka1, Lisa J Lapidus2.
Abstract
Observation of protein folding on submillisecond time scales requires specialized ultra-rapid mixers coupled to optical or chemical probes. Here we describe the protocol for employing a microfabricated mixer with a mixing time of 8 μs coupled to a UV confocal microscope. This instrument can detect Trp fluorescence and also excite hydroxyl radicals that label the folding protein which can be detected by mass spectrometry.Entities:
Keywords: Fast photochemical oxidation of proteins (FPOP); Microfluidic mixer; Protein folding; Trp fluorescence
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Year: 2022 PMID： 34845607 DOI： 10.1007/978-1-0716-1716-8_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745