Xuan Mei1,2, Dashuai Zhu1,2, Junlang Li1,2, Ke Huang1,2, Shiqi Hu1,2, Zhenhua Li1,2, Blanca López de Juan Abad1,2, Ke Cheng1,2,3. 1. Department of Molecular Biomedical Sciences and Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27695, USA. 2. Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. 3. Lead contact.
Abstract
BACKGROUND: Cardiac repair after heart injury remains a big challenge and current drug delivery to the heart is suboptimal. Repeated dosing of therapeutics is difficult due to the invasive nature of such procedures. METHODS: We developed a fluid-driven heart pouch with a memory-shaped microfabricated lattice structure inspired by origami. The origami structure allowed minimally invasive delivery of the pouch to the heart with two small incisions and can be refilled multiple times with the therapeutic of choice. FINDINGS: We tested the pouch's ability to deliver mesenchymal stem cells (MSCs) in a rodent model of acute myocardial infarction and demonstrated the feasibility of minimally invasive delivery in a swine model. The pouch's semi-permeable membrane successfully protected delivered cells from their surroundings, maintaining their viability while releasing paracrine factors to the infarcted site for cardiac repair. CONCLUSIONS: In summary, we developed a fluid-driven heart pouch with a memory-shaped microfabricated lattice structure inspired by origami. The origami structure allowed minimally invasive delivery of the pouch to the heart with two small incisions and can be refilled with the therapeutic of choice.
BACKGROUND: Cardiac repair after heart injury remains a big challenge and current drug delivery to the heart is suboptimal. Repeated dosing of therapeutics is difficult due to the invasive nature of such procedures. METHODS: We developed a fluid-driven heart pouch with a memory-shaped microfabricated lattice structure inspired by origami. The origami structure allowed minimally invasive delivery of the pouch to the heart with two small incisions and can be refilled multiple times with the therapeutic of choice. FINDINGS: We tested the pouch's ability to deliver mesenchymal stem cells (MSCs) in a rodent model of acute myocardial infarction and demonstrated the feasibility of minimally invasive delivery in a swine model. The pouch's semi-permeable membrane successfully protected delivered cells from their surroundings, maintaining their viability while releasing paracrine factors to the infarcted site for cardiac repair. CONCLUSIONS: In summary, we developed a fluid-driven heart pouch with a memory-shaped microfabricated lattice structure inspired by origami. The origami structure allowed minimally invasive delivery of the pouch to the heart with two small incisions and can be refilled with the therapeutic of choice.
Authors: Miles Montgomery; Samad Ahadian; Locke Davenport Huyer; Mauro Lo Rito; Robert A Civitarese; Rachel D Vanderlaan; Jun Wu; Lewis A Reis; Abdul Momen; Saeed Akbari; Aric Pahnke; Ren-Ke Li; Christopher A Caldarone; Milica Radisic Journal: Nat Mater Date: 2017-08-14 Impact factor: 43.841
Authors: Leo Timmers; José P S Henriques; Dominique P V de Kleijn; J Hans Devries; Hans Kemperman; Paul Steendijk; Cees W J Verlaan; Marjolein Kerver; Jan J Piek; Pieter A Doevendans; Gerard Pasterkamp; Imo E Hoefer Journal: J Am Coll Cardiol Date: 2009-02-10 Impact factor: 24.094
Authors: Junnan Tang; Jinqiang Wang; Ke Huang; Yanqi Ye; Teng Su; Li Qiao; Michael Taylor Hensley; Thomas George Caranasos; Jinying Zhang; Zhen Gu; Ke Cheng Journal: Sci Adv Date: 2018-11-28 Impact factor: 14.136