Vitor Batista Pinto1,2,3, Vinicius Costa Almeida4, Ítalo A Pereira-Lima5, Ellen Moura Vale6,7, Wagner L Araújo5, Vanildo Silveira6,7, José Marcelo Soriano Viana4. 1. Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil. vitorbp@uenf.br. 2. Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil. vitorbp@uenf.br. 3. Setor de Genômica e Proteômica. CBB, Unidade de Biologia Integrativa, UENF, Campos dos Goytacazes, RJ, Brazil. vitorbp@uenf.br. 4. Departamento de Biologia Geral, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil. 5. Departamento de Biologia Vegetal, Universidade Federal de Viçosa (UFV), Viçosa, MG, 36570-900, Brazil. 6. Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Av. Alberto Lamego, 2000, Campos dos Goytacazes, RJ, 28013-602, Brazil. 7. Setor de Genômica e Proteômica. CBB, Unidade de Biologia Integrativa, UENF, Campos dos Goytacazes, RJ, Brazil.
Abstract
MAIN CONCLUSION: Al responsive proteins are associated with starch, sucrose, and other carbohydrate metabolic pathways. Sucrose synthase is a candidate to Al tolerance. Al responses are regulated at transcriptional and post-transcriptional levels. Aluminum toxicity is one of the important abiotic stresses that affects worldwide crop production. The soluble form of aluminum (Al3+) inhibits root growth by altering water and nutrient uptake, a process that also reduces plant growth and development. Under long-term Al3+ exposure, plants can activate several tolerance mechanisms. To date, no reports of large-scale proteomic data concerning maize responses to this ion have been published. To investigate the post-transcriptional regulation in response to Al toxicity, we performed label-free quantitative proteomics for comparative analysis of two Al-contrasting popcorn inbred lines and an Al-tolerant commercial hybrid during 72 h under Al-stress conditions. A total of 489 differentially accumulated proteins (DAPs) were identified in the Al-sensitive inbred line, 491 in the Al-tolerant inbred line, and 277 in the commercial hybrid. Among them, 120 DAPs were co-expressed in both Al tolerant genotypes. Bioinformatics analysis indicated that starch, sucrose, and other components of carbohydrate metabolism and glycolysis/gluconeogenesis are the biochemical processes regulated in response to Al toxicity. Sucrose synthase accumulation and an increase in sucrose content and starch degradation suggest that these components may enhance popcorn tolerance to Al stress. The accumulation of citrate synthase suggests a key role for this enzyme in the detoxification process in the Al-tolerant inbred line. The integration of transcriptomic and proteomic data indicates that the Al tolerance response presents a complex regulatory network into the transcription and translation dynamics of popcorn root development.
MAIN CONCLUSION: Al responsive proteins are associated with starch, sucrose, and other carbohydrate metabolic pathways. Sucrose synthase is a candidate to Al tolerance. Al responses are regulated at transcriptional and post-transcriptional levels. Aluminum toxicity is one of the important abiotic stresses that affects worldwide crop production. The soluble form of aluminum (Al3+) inhibits root growth by altering water and nutrient uptake, a process that also reduces plant growth and development. Under long-term Al3+ exposure, plants can activate several tolerance mechanisms. To date, no reports of large-scale proteomic data concerning maize responses to this ion have been published. To investigate the post-transcriptional regulation in response to Al toxicity, we performed label-free quantitative proteomics for comparative analysis of two Al-contrasting popcorn inbred lines and an Al-tolerant commercial hybrid during 72 h under Al-stress conditions. A total of 489 differentially accumulated proteins (DAPs) were identified in the Al-sensitive inbred line, 491 in the Al-tolerant inbred line, and 277 in the commercial hybrid. Among them, 120 DAPs were co-expressed in both Al tolerant genotypes. Bioinformatics analysis indicated that starch, sucrose, and other components of carbohydrate metabolism and glycolysis/gluconeogenesis are the biochemical processes regulated in response to Al toxicity. Sucrose synthase accumulation and an increase in sucrose content and starch degradation suggest that these components may enhance popcorn tolerance to Al stress. The accumulation of citrate synthase suggests a key role for this enzyme in the detoxification process in the Al-tolerant inbred line. The integration of transcriptomic and proteomic data indicates that the Al tolerance response presents a complex regulatory network into the transcription and translation dynamics of popcorn root development.
Authors: Adam N Famoso; Randy T Clark; Jon E Shaff; Eric Craft; Susan R McCouch; Leon V Kochian Journal: Plant Physiol Date: 2010-06-10 Impact factor: 8.340
Authors: Joanna M Cross; Maria von Korff; Thomas Altmann; Linda Bartzetko; Ronan Sulpice; Yves Gibon; Natalia Palacios; Mark Stitt Journal: Plant Physiol Date: 2006-10-27 Impact factor: 8.340
Authors: Claudia T Guimaraes; Christiano C Simoes; Maria Marta Pastina; Lyza G Maron; Jurandir V Magalhaes; Renato C C Vasconcellos; Lauro J M Guimaraes; Ubiraci G P Lana; Carlos F S Tinoco; Roberto W Noda; Silvia N Jardim-Belicuas; Leon V Kochian; Vera M C Alves; Sidney N Parentoni Journal: BMC Genomics Date: 2014-02-24 Impact factor: 3.969
Authors: Eric W Deutsch; Attila Csordas; Zhi Sun; Andrew Jarnuczak; Yasset Perez-Riverol; Tobias Ternent; David S Campbell; Manuel Bernal-Llinares; Shujiro Okuda; Shin Kawano; Robert L Moritz; Jeremy J Carver; Mingxun Wang; Yasushi Ishihama; Nuno Bandeira; Henning Hermjakob; Juan Antonio Vizcaíno Journal: Nucleic Acids Res Date: 2016-10-18 Impact factor: 16.971
Authors: Shahid Khan; Vitor Batista Pinto; Antônio Teixeira do Amaral Júnior; Gabriel Moreno Bernardo Gonçalves; Caio Cézar Guedes Corrêa; Fernando Rafael Alves Ferreira; Guilherme Augusto Rodrigues de Souza; Eliemar Campostrini; Marta Simone Mendonça Freitas; Marlene Evangelista Vieira; Talles de Oliveira Santos; Valter Jário de Lima; Samuel Henrique Kamphorst; José Francisco Teixeira do Amaral; Freddy Mora-Poblete; Gonçalo Apolinário de Souza Filho; Vanildo Silveira Journal: Sci Rep Date: 2022-01-27 Impact factor: 4.996