Xiang Zhu1,2, Min Zhang1,2, Yongxin Xiao1,2, Tom Hsiang3, Ciyin Hu1,2, Junkai Li1,2. 1. Hubei Engineering Technology Center for Pest Forewarning and Management, College of Agriculture, Yangtze University, Jingzhou, China. 2. Institute of Pesticides, Yangtze University, Jingzhou, China. 3. School of Environmental Sciences, University of Guelph, Guelph, ON, Canada.
Abstract
BACKGROUND: Tobacco sore shin caused by Rhizoctonia solani Kühn is a major soil-borne fungal disease of tobacco, gradually causing infected stems to become thin and dry, leading to great losses to China's tobacco industry. Fungicides with phloem mobility are needed for application to foliage to effectively control root or vascular system pathogens. In this study, phenazine-1-carboxylic acid-valine conjugate (PCA-Val) with strong phloem mobility was tested for control of tobacco sore shin. In vitro fungicidal activity, systemicity, and in vivo efficacy of PCA-Val against R. solani in tobacco seedling were evaluated. RESULTS: In vitro fungicidal activity of PCA-L-Val against R. solani was lower than that of PCA or PCA-D-Val, but the in vivo protective activity and curative activity of PCA-L-Val was the highest among these chemicals tested. The systemicity tests in tobacco seedlings revealed that PCA did not possess phloem mobility, while PCA-L-Val and PCA-D-Val exhibited strong phloem mobility and could be transported and accumulated in the lower part of the seedling as well as throughout the phloem. In addition, we also found that, just like reported hormone amino acid conjugates, PCA-L-Val could be hydrolyzed by tobacco seedlings, to release free PCA. CONCLUSIONS: The current research results indicated that PCA-L-Val possess good phloem transport in tobacco and promising in vivo antifungal activity against R. solani, which can be used as a phloem-mobile fungicide against tobacco sore shin in production practice.
BACKGROUND: Tobacco sore shin caused by Rhizoctonia solani Kühn is a major soil-borne fungal disease of tobacco, gradually causing infected stems to become thin and dry, leading to great losses to China's tobacco industry. Fungicides with phloem mobility are needed for application to foliage to effectively control root or vascular system pathogens. In this study, phenazine-1-carboxylic acid-valine conjugate (PCA-Val) with strong phloem mobility was tested for control of tobacco sore shin. In vitro fungicidal activity, systemicity, and in vivo efficacy of PCA-Val against R. solani in tobacco seedling were evaluated. RESULTS: In vitro fungicidal activity of PCA-L-Val against R. solani was lower than that of PCA or PCA-D-Val, but the in vivo protective activity and curative activity of PCA-L-Val was the highest among these chemicals tested. The systemicity tests in tobacco seedlings revealed that PCA did not possess phloem mobility, while PCA-L-Val and PCA-D-Val exhibited strong phloem mobility and could be transported and accumulated in the lower part of the seedling as well as throughout the phloem. In addition, we also found that, just like reported hormone amino acid conjugates, PCA-L-Val could be hydrolyzed by tobacco seedlings, to release free PCA. CONCLUSIONS: The current research results indicated that PCA-L-Val possess good phloem transport in tobacco and promising in vivo antifungal activity against R. solani, which can be used as a phloem-mobile fungicide against tobacco sore shin in production practice.