| Literature DB >> 3479332 |
I L Mårtensson1, T Leanderson.
Abstract
Conditions for transfection of normal, freshly isolated and mitogen-stimulated spleen cells were established. A sharp optimum of plasmid at 10 micrograms per 1.5 X 10(7) transfected spleen cells per transfection was observed. The Ig promoter and the IgH enhancer activity was found to be restricted to cells of the B lineage also in normal, freshly isolated lymphocytes in vitro.Entities:
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Year: 1987 PMID: 3479332 DOI: 10.1002/eji.1830171017
Source DB: PubMed Journal: Eur J Immunol ISSN: 0014-2980 Impact factor: 5.532