The nitrification recovery capacity is the key to enhancing nitrogen removal in the AOA system at low temperatures.

Anaerobic/aerobic/anoxic (AOA) is suitable for advanced nitrogen removal of low C/N wastewater as an energy-saving process. Investigations of the temperature impact on the AOA process are critical to its application in cold regions or seasons. In this study, the nitrogen removal performance in AOA at low and room temperatures was investigated. The nitrification capacity of the AOA process was recovered at low temperature and the endogenous denitrification performance was enhanced by converting the partial aerobic zone into anoxic. At 15 °C, treating real municipal sewage with a low C/N ratio (3.36), TIN and NH4+-N removal efficiencies of 84.3 ± 6.6% and 97.4 ± 3.3% respectively, were achieved. The oxygen uptake rate test, quantitative PCR, and high-throughput sequencing results indicated that the activity and abundance of ammonia-oxidizing bacteria (AOB) increased at low temperature, which was the key for nitrification capacity recovery. Overall, the recoverability of nitrification capacity in the AOA system made advanced nitrogen removal possible at low temperatures.