| Literature DB >> 34784045 |
Frances Alexander1, Emily Brunt1, Holly Humphries1, Breeze Cavell1, Stephanie Leung1, Lauren Allen1, Rachel Halkerston1, Elodie Lesne1, Elizabeth Penn1, Stephen Thomas1, Andrew Gorringe1, Stephen Taylor2.
Abstract
Complement is a key component of functional immunological assays used to evaluate vaccine-mediated immunity to a range of bacterial and viral pathogens. However, standardization of these assays is complicated due to the availability of a human complement source that lacks existing antibodies acquired either through vaccination or natural circulation of the pathogen of interest. We have developed a method for depleting both IgG and IgM in 200 mL batches from pooled hirudin-derived human plasma by sequential affinity chromatography using a Protein G Sepharose column followed by POROS™ CaptureSelect™ IgM Affinity resin. The production of large IgG- and IgM-depleted batches of human plasma that retains total hemolytic and alternative pathway activities allows for improved assay standardization and comparison of immune responses in large clinical trials.Entities:
Keywords: Complement system; FPLC; Human plasma; IgG depletion; IgM depletion
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Year: 2022 PMID： 34784045 DOI： 10.1007/978-1-0716-1900-1_18
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745