Humberto Jácome-Santos1,2, Naira da Silva E Silva3, Renata Gonçalves Resende4, Helder Henrique Costa Pinheiro5, Luiz Fernando Almeida Machado6, Guilherme de Souza Silva7, Fernando de Oliveira Costa8, Igor Brasil-Costa6, Ana Cláudia Braga Amoras-Alves5, Ricardo Alves Mesquita8, Sérgio de Melo Alves-Junior5. 1. Laboratory of Pathology and Immunohistochemistry (LAPI), School of Dentistry, Universidade Federal do Pará (UFPA), Belém, PA, Brazil. humbertoufpa@yahoo.com.br. 2. Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. humbertoufpa@yahoo.com.br. 3. Department of Virology, Laboratory of Epstein-Barr Virus, Instituto Evandro Chagas (IEC), Belém, PA, Brazil. 4. Department of Stomatology & Oral and Maxillofacial Surgery, Hospital Metropolitano Odilon Behrens (HMOB), Belo Horizonte, MG, Brazil. 5. Laboratory of Pathology and Immunohistochemistry (LAPI), School of Dentistry, Universidade Federal do Pará (UFPA), Belém, PA, Brazil. 6. Laboratory of Virology, Universidade Federal do Pará (UFPA), Belém, PA, Brazil. 7. Hospital das Clínicas (HC), Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil. 8. Department of Oral Surgery and Pathology, School of Dentistry, Universidade Federal de Minas Gerais (UFMG), Belo Horizonte, MG, Brazil.
Abstract
OBJECTIVES: This study aimed to investigate the detection of Epstein-Barr virus (EBV) in oral squamous cell carcinoma (OSCC) and to verify the concordance of EBV-DNA frequency in subgingival sites and in the OSCC. METHODS: A cross-sectional study with 30 OSCC patients, aged from 44 to 88 years old, was conducted. Samples were collected in subgingival sites and at the OSCC, then submitted to DNA isolation, qPCR, and genotyping. Descriptive statistic was performed to report the frequency of EBV-DNA in all samples, and McNemar test was applied to verify the concordance among the EBV-DNA frequency in both sites. RESULTS: The individuals presented 62 years old in average, and the majority were male (66.6%). EBV-DNA was detected in 56.7% OSCC lesions. Among the subgroup of 19 dentate individuals, high concordance (73.7%) in both EBV-DNA detection and the absence in subgingival sites and OSCC was observed, and it was statistically significant (p < 0.05). CONCLUSIONS: We report the notable occurrence of EBV-DNA in OSCC; also, the presence of EBV in periodontal sites may contribute to find it in OSCC, although the possible contribution of EBV in the OSCC remains to be investigated. CLINICAL RELEVANCE: The identification of this easily accessible site of EBV latent infection may help to improve the patient's quality of life by maintenance of oral/periodontal health condition and preventing further possible disorders related to the virus, and also encourages new approaches for investigating EBV, periodontitis, and OSCC.
OBJECTIVES: This study aimed to investigate the detection of Epstein-Barr virus (EBV) in oral squamous cell carcinoma (OSCC) and to verify the concordance of EBV-DNA frequency in subgingival sites and in the OSCC. METHODS: A cross-sectional study with 30 OSCC patients, aged from 44 to 88 years old, was conducted. Samples were collected in subgingival sites and at the OSCC, then submitted to DNA isolation, qPCR, and genotyping. Descriptive statistic was performed to report the frequency of EBV-DNA in all samples, and McNemar test was applied to verify the concordance among the EBV-DNA frequency in both sites. RESULTS: The individuals presented 62 years old in average, and the majority were male (66.6%). EBV-DNA was detected in 56.7% OSCC lesions. Among the subgroup of 19 dentate individuals, high concordance (73.7%) in both EBV-DNA detection and the absence in subgingival sites and OSCC was observed, and it was statistically significant (p < 0.05). CONCLUSIONS: We report the notable occurrence of EBV-DNA in OSCC; also, the presence of EBV in periodontal sites may contribute to find it in OSCC, although the possible contribution of EBV in the OSCC remains to be investigated. CLINICAL RELEVANCE: The identification of this easily accessible site of EBV latent infection may help to improve the patient's quality of life by maintenance of oral/periodontal health condition and preventing further possible disorders related to the virus, and also encourages new approaches for investigating EBV, periodontitis, and OSCC.
Authors: David Chin; Glen M Boyle; Sandro Porceddu; David R Theile; Peter G Parsons; William B Coman Journal: Expert Rev Anticancer Ther Date: 2006-07 Impact factor: 4.512