| Literature DB >> 34773623 |
Wei Chang1, Yue Niu1, Mengna Yu1, Tian Li2,3, Jiana Li1,4,5, Kun Lu6,7,8.
Abstract
Real-time quantitative polymerase chain reaction (qPCR) is a powerful tool for analyzing and quantifying gene expression, and its primer design is the first and most important step. In order to improve the efficiency and effectiveness of primer design, we designed a database qPrimerDB, based on thermodynamics gene-specific for multispecies qPCR primers design.In this chapter, we explained the working principle of the database and detailed the step-by-step practical steps with examples. The valuable and time-saving qPrimerDB database is publicly accessible at http://biodb.swu.edu.cn/qprimerdb and will be routinely updated.Entities:
Keywords: Free online database; High-level efficiency; Primer design; Quantitative real-time polymerase chain reaction; qPrimerDB
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Year: 2022 PMID： 34773623 DOI： 10.1007/978-1-0716-1799-1_13
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745