Evaluation of the Inhibitory Effects of Pyridylpyrazole Derivatives on LPS-Induced PGE2 Productions and Nitric Oxide in Murine RAW 264.7 Macrophages.

A series of thirteen triarylpyrazole analogs were investigated as inhibitors of lipopolysaccharide (LPS)-induced prostaglandin E2 (PGE2) and nitric oxide (NO) production in RAW 264.7 macrophages. The target compounds 1a-m have first been assessed for cytotoxicity against RAW 264.7 macrophages to determine their non-cytotoxic concentration(s) for anti-inflammatory testing to make sure that the inhibition of PGE2 and NO production would not be caused by cytotoxicity. It was found that compounds 1f and 1m were the most potent PGE2 inhibitors with IC50 values of 7.1 and 1.1 μM, respectively. In addition, these compounds also showed inhibitory effects of 11.6% and 37.19% on LPS-induced NO production, respectively. The western blots analysis of COX-2 and iNOS showed that the PGE2 and NO inhibitory effect of compound 1m are attributed to inhibition of COX-2 and iNOS protein expression through inactivation of p38.

1. Introduction

Inflammation is considered as a part of our body’s defense mechanisms against invasive organisms. It represents an attempt to get rid of such harmful organisms through releasing antibacterial or antiviral from cells close to it to help the body fight against infection [1]. In addition, it enhances injured tissue healing facilitating the return of the cells to their normal conditions. Despite these beneficial effects, it could have harmful effects triggering a list of disorders such as cardiovascular disorders [2], tumors [3], inflammatory bowel syndrome [4], arthritis [5], pulmonary disorders [6], Alzheimer’s [7], etc.

In order to treat inflammation, it is crucial to understand the role of inflammatory mediators that directly contribute to inflammatory responses. Inflammatory mediators arise from plasma proteins or some types of cells such as mast cells, platelets, neutrophils, monocytes, and macrophages. They are triggered by bacterial toxins or host cell proteins. The inflammatory mediators bind to particular receptors on the target cells and enhance vascular permeability and neutrophil chemotaxis, induce smooth muscle contraction, directly affect enzymatic activity, produce pain, or induce oxidative damage. The majority of these chemical mediators have short lives but produce harmful effects [1]. The inflammatory chemical mediators are exemplified by vasoactive amines (e.g., histamine and 5-HT), eicosanoids (e.g., prostaglandins and leukotrienes), and cytokines (e.g., tumor necrosis factor (TNF) and interleukin-1 (IL-1)).

Cyclooxygenase-2 (COX-2) converts arachidonic acid into PGE2, which is the mediator of inflammation [8]. Limiting PGE2 production via inhibition of COX-2 protein expression and/or enzymatic activity is another useful approach for the treatment of inflammation. Moreover, nitric oxide (NO) has another considerable contribution to inflammation development (although it could produce anti-inflammatory effect under other normal physiological conditions) [9,10,11]. On the other hand, it acts as a proinflammatory mediator to induce localized inflammatory response due to elevated secretion in cases of abnormal conditions. Inducible nitric oxide synthase (iNOS) enzyme forms NO in case of inflammation. NO produces localized vasodilation at the site of inflammation, leading to edema [12]. Therefore, Similar to PGE2 production inhibition, decreasing NO production via iNOS enzymatic activity inhibition, and/or iNOS protein expression inhibition could be a beneficial avenue for the management of inflammation.

Many substituted pyrazole derivatives have been recently reported to possess anti-inflammatory activity [13,14,15,16]. In our study, we evaluated a series of substituted pyrazole derivatives with a structural likeness to celecoxib, a pyrazole-based anti-inflammatory agent (Figure 1) as inhibitors of LPS-induced NO and PGE2 productions. Vicinal diarylheterocycles such as celecoxib have been reported as COX-2-inhibiting anti-inflammatory agents. The presence of vicinal diarylpyrazole scaffold in the structures of our target compounds encouraged us to investigate their anti-inflammatory activity. Our target compounds 1a–m were previously reported as antiproliferative agents [17]. Moreover, compound I (Figure 1) possessing triarylpyrazole nucleus has been reported as inhibitor of PGE2 and NO release [18].

Figure 1

Structures of the lead compound I [18], celecoxib, and the target compounds 1a–m.

2. Results and Discussion

2.1. Chemistry

The final compounds 1a–m were synthesized via the pathway demonstrated in Scheme 1. 2-Chloro-5-methylphenol (2) was reacted with dimethyl sulfate/potassium carbonate to obtain methoxy derivative. The methyl group was then oxidized to carboxylic acid by potassium permanganate to 4-chloro-3-methoxy-benzoic acid. Esterification of the resulting acid by methanol and acetyl chloride yielded the corresponding methyl ester 3. Compound 3 was activated using a strong base; lithium bis(trimethylsilyl)amide (LiHMDS) followed by slow addition of 4-picoline gave the pyridine ketide intermediate 4. The reaction of compound 4 with dimethylformamide dimethylacetal (DMF-DMA) produced compound 5. After that, adding hydrazine monohydrate yielded the pyrazolyl intermediate 6. Interaction of compound 6 with meta-iodonitrobenzene at 90 °C in dimethyl sulfoxide gave the meta-nitrophenyl intermediate 7. Reduction of the NO2 group of 7 using Pd/C and hydrogen gas produced amino compound 8. Interaction of the amino intermediate 8 with chloroacetyl chloride or chloropropionyl chloride produced the corresponding amide intermediates 9a,b, respectively. Interaction of the terminal alkyl halide group of compounds 9a,b with (substituted) alicyclic amines gave the target compounds 1a–m [17]. The detailed experimental procedures and the spectral analysis charts are shown in the Supplementary File. Structures of compounds 1a–m and their cell viability results against RAW 264.7 cells are shown in Table 1.

Scheme 1

Reagents and conditions: (a) (i) (CH3)2SO4, K2CO3, acetone, reflux, 1 h, 95%; (ii) KMnO4, C5H5N, H2O, 50 °C, 24 h, then rt, 13 h, 90%; (iii) acetyl chloride, CH3OH, rt, 15 h, 85%; (b) 4-picoline, LiHMDS, THF, rt, overnight, 45%; (c) (i) DMF-DMA, rt, 18 h; (d) hydrazine monohydrate, C2H5OH, rt, overnight, 81%; (e) 1-iodo-4-nitrobenzene, K2CO3, CuI, L-proline, DMSO, 90 °C, 8 h, 86%; (f) H2, Pd/C, THF, rt, 2 h, 86%; (g) chloroacetyl chloride, or chloropropionyl chloride, TEA, CH2Cl2, −10 °C, 15 min, 65%; (h) appropriate amine derivative, TEA, CH2Cl2, rt, 1 h, 46–71%.

Table 1

Structures of compounds 1a–m and their cell viability results at 1 and 10 μM concentrations against murine RAW 264.7 macrophages.

Compound No.	n	R	Cell Viability (%)
			1 μM a	10 μM a
1a	1		99 ± 5.9	10 ± 1.2
1b	1		92 ± 4.5	65 ± 2.8
1c	1		78 ± 3.3	5 ± 0.8
1d	1		75 ± 2.9	3 ± 0.4
1e	1		87 ± 4.1	65 ± 4.1
1f	1		66 ± 1.8	107 ± 8.5
1g	2		87 ± 3.8	5 ± 1.1
1h	2		99 ± 2.8	75 ± 1.7
1i	2		99 ± 3.4	5 ± 1.8
1j	2		98 ± 3.1	5 ± 0.8
1k	2		89 ± 2.8	112 ± 7.6
1l	2		86 ± 3.7	34 ± 1.1
1m	2		90 ± 4.1	97 ± 3.9

a Data are presented as the means ± SDs of three independent experiments. Bold figures indicate non-cytotoxicity. * Indicates the site of connection to the main structure.

2.2. Biological Evaluation

Before screening the PGE2 and NO production inhibitory effects of the compounds, the compounds’ cytotoxicity was evaluated at 1 and 10 μM concentrations to make sure that the tested concentrations are safe enough and non-cytotoxic to avoid misleading results. All compounds were non-cytotoxic at 1 μM concentration, while by increasing concentration to 10 μM, all the target compounds except 1f, 1k, and 1m started showing cytotoxicity. These three compounds were found to be non-cytotoxic at 10 μM concentration (Figure 2). The three compounds possess N-benzylpiperazinyl, N-phenylpiperazinyl, and N-(4-fluorobenzyl)piperazinyl moieties, respectively. The piperidinyl and the morpholino moieties seem to be unfavorable to avoid cytotoxicity in this series of compounds.

Figure 2

Effects of compounds 1f (a), 1k (b), 1m (c), NS398, and L-NIL on LPS-induced RAW 264.7 cell viability at various concentrations (2.5, 5, or 10 μM).

Upon confirming the non-cytotoxicity of 10 μM concentration of these three derivatives against murine RAW 264.7 macrophages induced by LPS, compounds 1f, 1k, and 1m were tested for inhibitory effect against LPS-induced PGE2 production together with checking their cell viability. They have shown no cytotoxicity at these levels (2.5, 5, 10 μM) and good inhibition values against PGE2 production (Figure 2 and Figure 3). Among these selected derivatives, compounds 1f and 1m showed dose-dependent inhibition along with increasing its concentration (37.4 at 5 μM to 65.4% at 10 μM) for compound 1f, and 67% at 2.5 μM and 84.9% at 10 μM for compound 1m. Furthermore, the IC50 values of compounds 1f and 1m were 7.6 and 1.1 μM, respectively. This indicates that compound 1m with ethylene bridge was more active than compound 1f possessing methylene bridge. The fluorine atom of compound 1m might confer more lipophilicity that may result in more penetration inside the cell and hence better inhibition of PGE2 production. The fluorine atom can also add some more merits such as formation of an additional hydrogen bond with a hydrogen bond donor in the target protein and stronger hydrophobic interaction by fluorophenyl compared with unsubstituted phenyl. In addition, p-fluoro can prevent aromatic hydroxylation metabolic reaction and hence can elongate the duration of action [19]. Moreover, 1f and 1m were evaluated at 1 and 10 μM for inhibitory effects on LPS-induced NO production. It was found that compounds 1f and 1m showed inhibition values of 11.06% and 37.19%, respectively on LPS-induced NO production at 10 µM concentration (Table 2). Compound 1m is slightly more active than L-NIL at 10 µM.

Figure 3

Effects of compounds 1f (a), 1k (b), and 1m (c), and NS398 on LPS-stimulated PGE2 production in RAW 264.7 macrophages. # means significant difference from the negative control and *** means significant difference from the positive control.

Table 2

Inhibitory effects of compounds 1f and 1m against NO production in LPS-induced RAW 264.7 cells.

Compound	Inhibition Rate (%)
	1 (μM)	10 (μM)
1f	0	11.06 ± 1.5
1m	0	37.19 ± 3.4
L-NIL	3.9 ± 2.1	31.32 ± 2.9

Compounds 1f, 1k, and 1m were also tested for inhibitory effects against LPS-induced PGE2 production in addition to checking their cell viability. They have shown no cytotoxicity at these levels and good inhibition values against PGE2 production (Figure 2 and Figure 3). Among these selected derivatives, compounds 1f and 1m showed dose-dependent inhibition along with increasing its concentration (37.4% at 5 μM to 65.4% at 10 μM) for compound 1f, and 67% at 2.5 μM and 84.9% at 10 μM for compound 1m. Furthermore, the IC50 values of compounds 1f and 1m were 7.6 and 1.1 μM, respectively. This complies that compound 1m with ethylene bridge was more active than compound 1f possessing methylene bridge. The fluorine atom of compound 1m might confer more lipophilicity that may result in more penetration inside the cell and hence better inhibition of PGE2 production.

Furthermore, the most promising compound 1m was chosen for a more extensive investigation of its molecular mechanism(s) of action. It was tested for inhibitory effects on COX-2 and iNOS protein expressions (Figure 4). Compound 1m showed a concentration-dependent inhibitory effect against COX-2 and iNOS protein expression, especially at 10 µM concentration. Moreover, compound 1m markedly suppressed the phosphorylation of p38, a key molecule in regulating inflammation [20].

Figure 4

Effect of compound 1m on COX-2, iNOS, and p-p38 protein expression in LPS-induced RAW 264.7 macrophages.

3. Conclusions

Our target compounds were tested for potential cytotoxicity. We then selected the safest compounds for further investigations as PGE2 and NO production inhibitors in LPS-induced murine RAW 264.7 macrophages. The two tested compounds (1f and 1m) act more against PGE2 production than over NO. We identified a couple of potential PGE2 production inhibitory compounds, namely 1f and 1m. The most potent compound, 1m, exerted a strong inhibitory effect on PGE2 production with IC50 value of 1.1 μM and NO production with 37% at 10 μM. It produces these effects due to inhibition of both COX-2 and iNOS protein expression through inactivation of p38. Further structural optimization is needed in order to optimize activity.