| Literature DB >> 34766261 |
Abstract
Immunohistochemistry (IHC) is a highly sensitive protein detection technique developed using the principle of antigen-antibody binding reaction. With immunohistochemistry, it is possible to visualize the abundance, distribution, and localization of proteins in situ. This chapter discusses the standard protocols involved in IHC detection using chromogenic substrates, including pre-treatment of tissues, types of chromogenic substrates, and troubleshooting at various stages of the protocol.Entities:
Keywords: Antigen retrieval; Blocking; Chromogenic substrate; Detection; Fixation; Immunohistochemistry; Permeabilization; Troubleshooting
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Year: 2022 PMID： 34766261 DOI： 10.1007/978-1-0716-1771-7_1
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745