Qingfeng Liang1,2, Qihua Le1,3, Leying Wang2, Daniel Cordova1, Elfren Baclagon1, Sheyla González Garrido1, Mary Levin4, Yusheng Jin4, Chi-Hong Tseng5, Jianyu Rao4, Sophie X Deng1,6. 1. Stein Eye Institute, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA. 2. Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing, China. 3. Department of Ophthalmology, Eye, Ear, Nose & Throat Hospital of Fudan University, Shanghai, China. 4. Department of Pathology and Laboratory Medicine, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA. 5. Department of Medicine, Statistic Core-General Internal Medicine and Health Service Research, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA; and. 6. Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA.
Abstract
PURPOSE: The purpose of this study was to evaluate the expression of cytokeratin (K) 13 on the corneal surface and to validate its application in the diagnosis of limbal stem cell deficiency (LSCD). METHODS: This prospective comparative study included 26 corneal impression cytology (IC) specimens from patients diagnosed with LSCD. Twenty-three IC specimens from normal donors served as controls. K12 and K13 expression were detected on the IC specimens by immunohistochemistry study. The number of K12 + or K13 + cells in all areas of the IC was quantified using ImageJ software. RESULTS: The epithelial cells harvested from IC specimens from control corneas were all K12 + . In eyes with LSCD, K13 + and K12 + /K13 + cells accounted for 93.8% and 2.6%, respectively, in the cornea. In eyes with sectoral LSCD, the median number of K13 + cells in the clinically affected area was higher than that in the unaffected area (810.0 vs. 115.0 cells/mm 2 ; P < 0.001). No significant correlation was found between the LSCD severity and the number of K12 + cells (r = -0.284, P = 0.16) or K13 + cells (r = -0.011, P = 0.95). The presence of at least 16 K13 + cells/mm 2 was suggestive of LSCD. CONCLUSIONS: Identification of K13 + cells on IC specimens provides a simple and reliable method to detect conjunctival epithelial cells on the cornea. K13 is a marker for diagnosing LSCD and localizing the involved area in sectoral LSCD.
PURPOSE: The purpose of this study was to evaluate the expression of cytokeratin (K) 13 on the corneal surface and to validate its application in the diagnosis of limbal stem cell deficiency (LSCD). METHODS: This prospective comparative study included 26 corneal impression cytology (IC) specimens from patients diagnosed with LSCD. Twenty-three IC specimens from normal donors served as controls. K12 and K13 expression were detected on the IC specimens by immunohistochemistry study. The number of K12 + or K13 + cells in all areas of the IC was quantified using ImageJ software. RESULTS: The epithelial cells harvested from IC specimens from control corneas were all K12 + . In eyes with LSCD, K13 + and K12 + /K13 + cells accounted for 93.8% and 2.6%, respectively, in the cornea. In eyes with sectoral LSCD, the median number of K13 + cells in the clinically affected area was higher than that in the unaffected area (810.0 vs. 115.0 cells/mm 2 ; P < 0.001). No significant correlation was found between the LSCD severity and the number of K12 + cells (r = -0.284, P = 0.16) or K13 + cells (r = -0.011, P = 0.95). The presence of at least 16 K13 + cells/mm 2 was suggestive of LSCD. CONCLUSIONS: Identification of K13 + cells on IC specimens provides a simple and reliable method to detect conjunctival epithelial cells on the cornea. K13 is a marker for diagnosing LSCD and localizing the involved area in sectoral LSCD.
Authors: Aline Lütz de Araújo; José Reinaldo da Silva Ricardo; Vivian Naomi Sakai; Jeison Nadai de Barros; José Álvaro Pereira Gomes Journal: Arq Bras Oftalmol Date: 2013-10 Impact factor: 0.872
Authors: Sophie X Deng; Friedrich Kruse; José A P Gomes; Clara C Chan; Sheraz Daya; Reza Dana; Francisco C Figueiredo; Shigeru Kinoshita; Paolo Rama; Virender Sangwan; Allan R Slomovic; Donald Tan Journal: Cornea Date: 2020-10 Impact factor: 2.651
Authors: T T Sun; R Eichner; W G Nelson; S C Tseng; R A Weiss; M Jarvinen; J Woodcock-Mitchell Journal: J Invest Dermatol Date: 1983-07 Impact factor: 8.551