Md Mosiur Rahman1,2, Md Ruhul Quddus3, Md Omar Ali2, Rong Liu1, Mengwei Li1, Xin Yan1, Guan Li1, Yishan Ji1, Md Monoar Hossain1, Chenyu Wang1, Ashutosh Sarker4, Tao Yang5, Xuxiao Zong6. 1. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China. 2. Pulses Research Center, Bangladesh Agricultural Research Institute (BARI), Gazipur, Bangladesh. 3. Hybrid Rice Division, Bangladesh Rice Research Institute (BRRI), Gazipur, Bangladesh. 4. International Center for Agricultural Research in the Dry Areas (ICARDA), New Delhi, 110012, India. A.SARKER@CGIAR.ORG. 5. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China. yangtao02@caas.cn. 6. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China. zongxuxiao@caas.cn.
Abstract
BACKGROUND: The demand for grass peas (Lathyrus sativus L.) had increased as high nutritional safe food, but most of the accessions of South Asia and Africa had low grain harvest. Therefore, this study had been undertaken to collect grass pea germplasm for boosting yields and quality improvement. METHODS AND RESULTS: In this study, 400 accessions of grass pea from different geographical regions had characterized by using 56 Simple Sequences Repeat (SSRs) markers. In total 253 alleles were detected, the maximum and minimum polymorphic information content (PIC) indices were 0.70 and 0.34 found in markers G17922 and G18078, correspondingly. The germplasm was split into two main and one sub-group by cluster assay, by SSR assay, and three populations by model-based population structure analysis (Pop1, Pop2 and admixed). Neighbors joining tree assay showed the tested germplasm highly diverse in structure. Three-dimensional principal components analysis (PCA) and two dimensional principles coordinate analysis (PCoA) exhibited two main and one admixed group (P1, P2 and P1P2). In addition, FST population value of pairwise mean and analysis of molecular variance (AMOVA) showed high population structure across all pairs of populations on an average 0.1710 advocating all population structure categories varied significantly. The average predictable heterozygosity distant was 0.4472-0.4542 in same cluster for the individuals. CONCLUSION: Discovery from this study revealed SSR markers based polymorphic bands showed in the diversified grasspea germplasm which might be utilized as genetic resource of a breeding scheme and prospective uses for mapping analyses of recombinant inbred lines (RIL).
BACKGROUND: The demand for grass peas (Lathyrus sativus L.) had increased as high nutritional safe food, but most of the accessions of South Asia and Africa had low grain harvest. Therefore, this study had been undertaken to collect grass pea germplasm for boosting yields and quality improvement. METHODS AND RESULTS: In this study, 400 accessions of grass pea from different geographical regions had characterized by using 56 Simple Sequences Repeat (SSRs) markers. In total 253 alleles were detected, the maximum and minimum polymorphic information content (PIC) indices were 0.70 and 0.34 found in markers G17922 and G18078, correspondingly. The germplasm was split into two main and one sub-group by cluster assay, by SSR assay, and three populations by model-based population structure analysis (Pop1, Pop2 and admixed). Neighbors joining tree assay showed the tested germplasm highly diverse in structure. Three-dimensional principal components analysis (PCA) and two dimensional principles coordinate analysis (PCoA) exhibited two main and one admixed group (P1, P2 and P1P2). In addition, FST population value of pairwise mean and analysis of molecular variance (AMOVA) showed high population structure across all pairs of populations on an average 0.1710 advocating all population structure categories varied significantly. The average predictable heterozygosity distant was 0.4472-0.4542 in same cluster for the individuals. CONCLUSION: Discovery from this study revealed SSR markers based polymorphic bands showed in the diversified grasspea germplasm which might be utilized as genetic resource of a breeding scheme and prospective uses for mapping analyses of recombinant inbred lines (RIL).
Authors: Michael J Thomson; Endang M Septiningsih; Fatimah Suwardjo; Tri J Santoso; Tiur S Silitonga; Susan R McCouch Journal: Theor Appl Genet Date: 2006-11-29 Impact factor: 5.699