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Literature DB >> 3474152

Activated H-ras transforms rat intestinal epithelial cells with expression of alpha-TGF.

Abstract

We describe malignant transformation of cultured rat intestinal epithelial cells (IEC-18) by transfection with the activated human H-ras gene cloned from the EJ bladder carcinoma. Transformed cells showed a marked morphological change, expressed high levels of the transfected H-ras gene, were able to grow in agar and expressed antigenic markers identical with parental IEC-18 cells. When injected into syngeneic rats these cells formed rapidly growing tumors expressing the same intestinal-specific antigenic markers as the injected cells. Parallel to the high expression of H-ras mRNA in the transformants we document overexpression of rat alpha-TGF mRNA.

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Year: 1987 PMID： 3474152 DOI： 10.1016/0014-4827(87)90308-9

Source DB: PubMed Journal: Exp Cell Res ISSN： 0014-4827 Impact factor: 3.905

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Cited

10 in total

1. Collateral expression of proangiogenic and tumorigenic properties in intestinal epithelial cell variants selected for resistance to anoikis.

Authors: J Rak; Y Mitsuhashi; C Sheehan; J K Krestow; V A Florenes; J Filmus; R S Kerbel
Journal: Neoplasia Date: 1999-04 Impact factor: 5.715

2. Normal rat intestinal cells IEC-18: characterization and transfection with immortalizing oncogenes.

Authors: O Vincentini; C Ciotta; M Bignami; A Stammati; F Zucco
Journal: Cytotechnology Date: 1996-01 Impact factor: 2.058

3. Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine.

Authors: J Filmus; J G Church; R N Buick
Journal: Mol Cell Biol Date: 1988-10 Impact factor: 4.272

4. Impact of oncogenes in tumor angiogenesis: mutant K-ras up-regulation of vascular endothelial growth factor/vascular permeability factor is necessary, but not sufficient for tumorigenicity of human colorectal carcinoma cells.

Authors: F Okada; J W Rak; B S Croix; B Lieubeau; M Kaya; L Roncari; S Shirasawa; T Sasazuki; R S Kerbel
Journal: Proc Natl Acad Sci U S A Date: 1998-03-31 Impact factor: 11.205

10. Massive programmed cell death in intestinal epithelial cells induced by three-dimensional growth conditions: suppression by mutant c-H-ras oncogene expression.

Authors: J Rak; Y Mitsuhashi; V Erdos; S N Huang; J Filmus; R S Kerbel
Journal: J Cell Biol Date: 1995-12 Impact factor: 10.539

10 in total

Activated H-ras transforms rat intestinal epithelial cells with expression of alpha-TGF.

1. Collateral expression of proangiogenic and tumorigenic properties in intestinal epithelial cell variants selected for resistance to anoikis.

2. Normal rat intestinal cells IEC-18: characterization and transfection with immortalizing oncogenes.

3. Isolation of a cDNA corresponding to a developmentally regulated transcript in rat intestine.

4. Impact of oncogenes in tumor angiogenesis: mutant K-ras up-regulation of vascular endothelial growth factor/vascular permeability factor is necessary, but not sufficient for tumorigenicity of human colorectal carcinoma cells.

Review 5. Oncogenes as inducers of tumor angiogenesis.

6. Fendiline inhibits K-Ras plasma membrane localization and blocks K-Ras signal transmission.

7. Syndecan-1 is up-regulated in ras-transformed intestinal epithelial cells.

8. Oncogenic RAS drives the CRAF-dependent extracellular vesicle uptake mechanism coupled with metastasis.

9. Sos-mediated cross-activation of wild-type Ras by oncogenic Ras is essential for tumorigenesis.

10. Massive programmed cell death in intestinal epithelial cells induced by three-dimensional growth conditions: suppression by mutant c-H-ras oncogene expression.