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Literature DB >> 347400

Preparation and properties of insolubilized restriction endonucleases.

Y H Lee, R W Blakesley, L A Smith, J G Chirikjian.

Abstract

Type II restriction endonucleases Bam HI and Eco RI were covalently coupled to Sepharose. These insolubilized enzymes generated fragment patterns for several viral DNAs identical to those produced by the respective free enzymes. Conditions for optimal activity were similar for both bound and unbound forms of the enzymes. Insolubilization improved thermal stability of Bam HI and Eco RI. The bound enzyme can be recovered from reaction mixtures and reused several times. Upon storage at 4 degrees C, coupled endonucleases remained stable for several months.

Entities: Chemical Disease

Mesh：

Substances：

Year: 1978 PMID： 347400 PMCID： PMC342015 DOI： 10.1093/nar/5.3.679

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. Preparation and properties of water-insoluble derivatives of trypsin.

Authors: A BAR-ELI; E KATCHALSKI
Journal: J Biol Chem Date: 1963-05 Impact factor: 5.157

2. Specificity of substrate recognition by the EcoRI restriction endonuclease.

Authors: B Polisky; P Greene; D E Garfin; B J McCarthy; H M Goodman; H W Boyer
Journal: Proc Natl Acad Sci U S A Date: 1975-09 Impact factor: 11.205

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors: P A Sharp; B Sugden; J Sambrook
Journal: Biochemistry Date: 1973-07-31 Impact factor: 3.162

4. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.

Authors: K J Danna; G H Sack; D Nathans
Journal: J Mol Biol Date: 1973-08-05 Impact factor: 5.469

5. Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.

Authors: J H Middleton; M H Edgell; C A Hutchison
Journal: J Virol Date: 1972-07 Impact factor: 5.103

6. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

Authors: A C Peacock; C W Dingman
Journal: Biochemistry Date: 1968-02 Impact factor: 3.162

Review 7. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

Preparation and properties of insolubilized restriction endonucleases.

1. Preparation and properties of water-insoluble derivatives of trypsin.

2. Specificity of substrate recognition by the EcoRI restriction endonuclease.

3. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

4. Studies of simian virus 40 DNA. VII. A cleavage map of the SV40 genome.

5. Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.

6. Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gels.

Review 7. Restriction endonucleases.

8. A simple method for DNA restriction site mapping.

9. Duplex regions in "single-stranded" phiX174 DNA are cleaved by a restriction endonuclease from Haemophilus aegyptius.

10. The preparation and properties of ficin chemically attached to carboxymethylcellulose.

1. Effect of glutaraldehyde on the activity of some DNA restriction endonucleases.

2. Immobilization of the restriction endonucleases PvuII and HindIII.

3. Biospecific fractionation matrices for sequence specific endonucleases.