Irina Stepanov1,2, Prakash C Gupta3, Mark Parascandola4, Katrina Yershova1, Vipin Jain1, Gauri Dhumal3, Dorothy K Hatsukami1. 1. Masonic Cancer Center, University of Minnesota, Minneapolis, Minnesota, USA. 2. Division of Environmental Health Sciences, University of Minnesota, Minneapolis, Minnesota, USA. 3. Healis-Sekhsaria Institute for Public Health, Mumbai, Maharashtra, India. 4. Tobacco Control Research Branch, National Cancer Institute, Bethesda, Maryland, USA.
Abstract
OBJECTIVES: Worldwide, smokeless tobacco products vary greatly in their formulations and chemical composition. Understanding of toxic and carcinogenic constituent variations in such products can provide valuable insights for the development of effective tobacco control policies. In this study, we applied a standardized protocol to collect and analyze smokeless products sold in Mumbai, India. METHODS: Tobacco products were purchased at three markets in Mumbai, using standardized protocol for sample collection, labeling, and storage. Moisture content, pH, total and unprotonated nicotine, and five tobacco-specific N-nitrosamines (TSNA) were analyzed by validated methods. RESULTS: We purchased 39 samples representing eight varieties of manufactured and vendor-made smokeless tobacco products. Total nicotine ranged from 5.3 to 57.8 mg/g dry weight. Unprotonated nicotine content varied from 0.13% to 99.8% of total nicotine. Total TSNA content ranged from 0.17 to 81.0 μg/g dry weight. When expressed per wet weight of product, unprotonated nicotine varied more than 300-fold and TSNA content varied more than 650-fold across the products. Substantial vendor-to-vendor variations were also observed. CONCLUSIONS: Our findings emphasize the critical need for systematic smokeless tobacco surveillance in India, to improve understanding of exposures and cancer risks in users of these products.
OBJECTIVES: Worldwide, smokeless tobacco products vary greatly in their formulations and chemical composition. Understanding of toxic and carcinogenic constituent variations in such products can provide valuable insights for the development of effective tobacco control policies. In this study, we applied a standardized protocol to collect and analyze smokeless products sold in Mumbai, India. METHODS: Tobacco products were purchased at three markets in Mumbai, using standardized protocol for sample collection, labeling, and storage. Moisture content, pH, total and unprotonated nicotine, and five tobacco-specific N-nitrosamines (TSNA) were analyzed by validated methods. RESULTS: We purchased 39 samples representing eight varieties of manufactured and vendor-made smokeless tobacco products. Total nicotine ranged from 5.3 to 57.8 mg/g dry weight. Unprotonated nicotine content varied from 0.13% to 99.8% of total nicotine. Total TSNA content ranged from 0.17 to 81.0 μg/g dry weight. When expressed per wet weight of product, unprotonated nicotine varied more than 300-fold and TSNA content varied more than 650-fold across the products. Substantial vendor-to-vendor variations were also observed. CONCLUSIONS: Our findings emphasize the critical need for systematic smokeless tobacco surveillance in India, to improve understanding of exposures and cancer risks in users of these products.
Authors: Stephen B Stanfill; Gregory N Connolly; Liqin Zhang; Lily T Jia; Jack E Henningfield; Patricia Richter; Tameka S Lawler; Olalekan A Ayo-Yusuf; David L Ashley; Clifford H Watson Journal: Tob Control Date: 2010-11-25 Impact factor: 7.552
Authors: Jiachen Zhou; Dominique S Michaud; Scott M Langevin; Michael D McClean; Melissa Eliot; Karl T Kelsey Journal: Int J Cancer Date: 2012-10-11 Impact factor: 7.396