Chang Seok Oh1, Jong Ha Hong2, Jong Yil Chai3,4, Mi Kyung Song5, Ho-Jin Jang6, Min Seo7, Dong Hoon Shin8. 1. Department of Mortuary Science, College of Bio-Convergence, Eulji University, 553, Sanseongdae-ro, Sujeong-gu, Seongnam-si, Gyeonggi-do, 13135, South Korea. 2. Institute of Korean Archaeology and Ancient History, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447, South Korea. 3. Institute of Parasitic Diseases, Korea Association of Health Promotion, Seoul, 07649, South Korea. 4. Department of Tropical Medicine and Parasitology, Seoul National University College of Medicine, 103, Daehak-ro, Chongno-gu, Seoul, 03080, South Korea. 5. Department of Fashion Design and Marketing, Seoul Women's University, 621, Hwarang-ro, Nowon-gu, Seoul, 01797, South Korea. 6. Gaon Research Institute of Cultural Properties, 1311, Daegaya-ro, Daegaya-eup, Goryeong-gun, Gyeongsangbuk-do, 40141, South Korea. 7. Department of Parasitology, Dankook University College of Medicine, 119, Dandae-ro, Dongnam-gu, Cheonan-si, Chungcheongnam-do, 31116, South Korea. bbbenji@naver.com. 8. Institute of Forensic and Anthropological Science, Seoul National University College of Medicine, 103, Daehak-ro, Chongno-gu, Seoul, 03080, South Korea. cuteminjae@gmail.com.
Abstract
PURPOSE: Metagonimiasis, commonly seen in East Asian countries, is a parasitic disorder caused by definitive hosts' ingestion of undercooked freshwater fishes. Recently, genetic analysis has proved 28S rRNA and cytochrome c oxidase subunit I (COI) mtDNA gene to be a successful marker differentiating species of the genus Metagonimus. In the present study, using specimens from the newly discovered Joseon Dynasty human remains of Goryeong, we obtained updated genetic data on genus Metagonimus, which was also prevalent during the Joseon period. METHODS: The ancient DNA (aDNA) was retrieved from the coprolite sample of the seventeenth century, half-mummified individual discovered at Goryeong Country, South Korea. Cloning and sequencing were performed on PCR-amplified amplicons for M. yokogawai 28S rRNA and COI mtDNA gene. The consensus sequences were used for species identification and phylogenetic analysis using NCBI/BLAST and MEGA X software. RESULTS: Based on the COI mtDNA gene region, the Goryeong sequence was confirmed as belonging to M. yokogawai, as it was shown to form a separate cluster with other M. yokogawai taxa that are distinct also from M. takahashii and M. miyatai. CONCLUSION: In a series of our genetic analyses on genus Metagonimus using samples retrieved from Joseon-period cases, aDNA sequences of genus Metagonimus revealed in South Korea thus far are those of M. yokogawai, but not of M. miyatai or M. takahashii yet.
PURPOSE: Metagonimiasis, commonly seen in East Asian countries, is a parasitic disorder caused by definitive hosts' ingestion of undercooked freshwater fishes. Recently, genetic analysis has proved 28S rRNA and cytochrome c oxidase subunit I (COI) mtDNA gene to be a successful marker differentiating species of the genus Metagonimus. In the present study, using specimens from the newly discovered Joseon Dynasty human remains of Goryeong, we obtained updated genetic data on genus Metagonimus, which was also prevalent during the Joseon period. METHODS: The ancient DNA (aDNA) was retrieved from the coprolite sample of the seventeenth century, half-mummified individual discovered at Goryeong Country, South Korea. Cloning and sequencing were performed on PCR-amplified amplicons for M. yokogawai 28S rRNA and COI mtDNA gene. The consensus sequences were used for species identification and phylogenetic analysis using NCBI/BLAST and MEGA X software. RESULTS: Based on the COI mtDNA gene region, the Goryeong sequence was confirmed as belonging to M. yokogawai, as it was shown to form a separate cluster with other M. yokogawai taxa that are distinct also from M. takahashii and M. miyatai. CONCLUSION: In a series of our genetic analyses on genus Metagonimus using samples retrieved from Joseon-period cases, aDNA sequences of genus Metagonimus revealed in South Korea thus far are those of M. yokogawai, but not of M. miyatai or M. takahashii yet.
Authors: Martin Jensen Søe; Peter Nejsum; Frederik Valeur Seersholm; Brian Lund Fredensborg; Ruben Habraken; Kirstine Haase; Mette Marie Hald; Rikke Simonsen; Flemming Højlund; Louise Blanke; Inga Merkyte; Eske Willerslev; Christian Moliin Outzen Kapel Journal: PLoS One Date: 2018-04-25 Impact factor: 3.240