Literature DB >> 347245

Cloning of a restriction fragment of phage mu DNA coding for early functions.

W Schumann, E G Bade, H Delius, P Hübert.   

Abstract

The DNA of an E. coli K12 strain harboring ten wildtype Mu prophages was restricted with endonuclease EcoRI, and the fragments ligated into the plasmid vector pMB9. Upon transformation of a strain carrying a heat inducible (Mu cts62) prophage, one temperature-resistant transformant was isolated. This transformant strain harbors the hybrid plasmid pKN001, containing the EcoRI.C fragment of Mu DNA as shown by restriction and heteroduplex analysis. Stable transformants of pKN001 are immune to superinfection with phage Mu. Transformation of Mu sensitive bacteria with pKN001 results in killing of the recipients (10(-4) surviving bacteria). The killing function is not expressed upon transformation of Mu-immune (lysogenic) bacteria.

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Year:  1978        PMID： 347245     DOI： 10.1007/bf00275127

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  9 in total

Review 1.  Molecular biology of bacteriophage mu.

Authors:  M M Howe; E G Bade
Journal:  Science       Date:  1975-11-14       Impact factor: 47.728

2.  Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.

Authors:  M Thomas; R W Davis
Journal:  J Mol Biol       Date:  1975-01-25       Impact factor: 5.469

3.  Model for the enchancement of lambde-gal integration into partially induced Mu-1 lysogens.

Authors:  M Faelen; A Toussaint; J De Lafonteyne
Journal:  J Bacteriol       Date:  1975-03       Impact factor: 3.490

4.  Participation of Escherichia coli DNA in the replication of temperate bacteriophage Mu1.

Authors:  W Schroeder; E G Bade; H Delius
Journal:  Virology       Date:  1974-08       Impact factor: 3.616

5.  Connecting two unrelated DNA sequences with a Mu dimer.

Authors:  A Toussaint; M Faelen
Journal:  Nat New Biol       Date:  1973-03-07

6.  Mapping the DNA fragments produced by cleavage by lambda DNA with endonuclease RI.

Authors:  B Allet; P G Jeppesen; K J Katagiri; H Delius
Journal:  Nature       Date:  1973-01-12       Impact factor: 49.962

7.  Construction of a colicin E1-R factor composite plasmid in vitro: means for amplification of deoxyribonucleic acid.

Authors:  T Tanaka; B Weisblum
Journal:  J Bacteriol       Date:  1975-01       Impact factor: 3.490

8.  Biochemical method for inserting new genetic information into DNA of Simian Virus 40: circular SV40 DNA molecules containing lambda phage genes and the galactose operon of Escherichia coli.

Authors:  D A Jackson; R H Symons; P Berg
Journal:  Proc Natl Acad Sci U S A       Date:  1972-10       Impact factor: 11.205

9.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205
  9 in total
  5 in total

1.  Origin and binding specificity of protein(s) coded for by Mu prophages.

Authors:  W Schumann; C Westphal; E G Bade; L Holzer
Journal:  Mol Gen Genet       Date:  1979-06-07

2.  Construction of an HpaI and HindII plasmid vector allowing direct selection of transformants harboring recombinant plasmids.

Authors:  W Schumann
Journal:  Mol Gen Genet       Date:  1979-07-13

3.  Inhibition of bacterial segregation by early functions of phage mu and association of replication protein B with the inner cell membrane.

Authors:  C Boeckh; E G Bade; H Delius; J N Reeve
Journal:  Mol Gen Genet       Date:  1986-03

4.  In vitro constructed plasmids containing both ends of bacteriophage Mu DNA express phage functions.

Authors:  W Schumann; E G Bade
Journal:  Mol Gen Genet       Date:  1979-01-16

5.  Plasmid vectors derived from phage Mu allow direct selection of transformants containing cloned HindIII and PstI fragments.

Authors:  W Schumann; C Lögl
Journal:  Mol Gen Genet       Date:  1980
  5 in total

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