Literature DB >> 34709612

Identification of Essential Genes Using Sequential CRISPR and siRNA Screens.

Luke DeHart1, Oliver P Yockey1, Jesse Bakke2.   

Abstract

Genome-wide CRISPR and siRNA screening methodologies are powerful tools that are aptly suited to the discovery of essential genes. In this chapter, we outline our methods to conduct sequential CRISPR and siRNA screens to quickly and efficiently identify essential genes within a collection of cell lines. The utilization of both screening methodologies provides a pipeline that minimizes costs and time while enabling the robust detection of candidate genes.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  CRISPR; Essential genes; siRNA

Mesh：

Substances：

Year:  2022        PMID： 34709612     DOI： 10.1007/978-1-0716-1720-5_5

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  16 in total

1.  Essential gene profiles in breast, pancreatic, and ovarian cancer cells.

Authors:  Richard Marcotte; Kevin R Brown; Fernando Suarez; Azin Sayad; Konstantina Karamboulas; Paul M Krzyzanowski; Fabrice Sircoulomb; Mauricio Medrano; Yaroslav Fedyshyn; Judice L Y Koh; Dewald van Dyk; Bodhana Fedyshyn; Marianna Luhova; Glauber C Brito; Franco J Vizeacoumar; Frederick S Vizeacoumar; Alessandro Datti; Dahlia Kasimer; Alla Buzina; Patricia Mero; Christine Misquitta; Josee Normand; Maliha Haider; Troy Ketela; Jeffrey L Wrana; Robert Rottapel; Benjamin G Neel; Jason Moffat
Journal:  Cancer Discov       Date:  2011-12-29       Impact factor: 39.397

2.  Genome-wide CRISPR screen identifies HNRNPL as a prostate cancer dependency regulating RNA splicing.

Authors:  Teng Fei; Yiwen Chen; Tengfei Xiao; Wei Li; Laura Cato; Peng Zhang; Maura B Cotter; Michaela Bowden; Rosina T Lis; Shuang G Zhao; Qiu Wu; Felix Y Feng; Massimo Loda; Housheng Hansen He; X Shirley Liu; Myles Brown
Journal:  Proc Natl Acad Sci U S A       Date:  2017-06-13       Impact factor: 11.205

3.  Genome-scale CRISPR-Cas9 knockout screening in human cells.

Authors:  Ophir Shalem; Neville E Sanjana; Ella Hartenian; Xi Shi; David A Scott; Tarjei Mikkelson; Dirk Heckl; Benjamin L Ebert; David E Root; John G Doench; Feng Zhang
Journal:  Science       Date:  2013-12-12       Impact factor: 47.728

4.  Genome-wide CRISPR screen in a mouse model of tumor growth and metastasis.

Authors:  Sidi Chen; Neville E Sanjana; Kaijie Zheng; Ophir Shalem; Kyungheon Lee; Xi Shi; David A Scott; Jun Song; Jen Q Pan; Ralph Weissleder; Hakho Lee; Feng Zhang; Phillip A Sharp
Journal:  Cell       Date:  2015-03-05       Impact factor: 41.582

5.  High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.

Authors:  Traver Hart; Megha Chandrashekhar; Michael Aregger; Zachary Steinhart; Kevin R Brown; Graham MacLeod; Monika Mis; Michal Zimmermann; Amelie Fradet-Turcotte; Song Sun; Patricia Mero; Peter Dirks; Sachdev Sidhu; Frederick P Roth; Olivia S Rissland; Daniel Durocher; Stephane Angers; Jason Moffat
Journal:  Cell       Date:  2015-11-25       Impact factor: 41.582

6.  A Genome-wide CRISPR Death Screen Identifies Genes Essential for Oxidative Phosphorylation.

Authors:  Jason D Arroyo; Alexis A Jourdain; Sarah E Calvo; Carmine A Ballarano; John G Doench; David E Root; Vamsi K Mootha
Journal:  Cell Metab       Date:  2016-09-22       Impact factor: 27.287

7.  Identification of PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide) as an essential gene for colorectal cancer (CRCs) cells.

Authors:  Shangfeng Sun; Shuguang Cheng; Yunxiao Zhu; Peng Zhang; Ning Liu; Tong Xu; Chao Sun; Yanfeng Lv
Journal:  Gene       Date:  2016-03-15       Impact factor: 3.688

8.  Improved vectors and genome-wide libraries for CRISPR screening.

Authors:  Neville E Sanjana; Ophir Shalem; Feng Zhang
Journal:  Nat Methods       Date:  2014-08       Impact factor: 28.547

9.  An integrated genomic screen identifies LDHB as an essential gene for triple-negative breast cancer.

Authors:  Mark L McCleland; Adam S Adler; Yonglei Shang; Thomas Hunsaker; Tom Truong; David Peterson; Eric Torres; Li Li; Benjamin Haley; Jean-Philippe Stephan; Marcia Belvin; Georgia Hatzivassiliou; Elizabeth M Blackwood; Laura Corson; Marie Evangelista; Jiping Zha; Ron Firestein
Journal:  Cancer Res       Date:  2012-11-08       Impact factor: 12.701

10.  A Genome-wide CRISPR Screen in Primary Immune Cells to Dissect Regulatory Networks.

Authors:  Oren Parnas; Marko Jovanovic; Thomas M Eisenhaure; Rebecca H Herbst; Atray Dixit; Chun Jimmie Ye; Dariusz Przybylski; Randall J Platt; Itay Tirosh; Neville E Sanjana; Ophir Shalem; Rahul Satija; Raktima Raychowdhury; Philipp Mertins; Steven A Carr; Feng Zhang; Nir Hacohen; Aviv Regev
Journal:  Cell       Date:  2015-07-16       Impact factor: 41.582
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