Literature DB >> 34709511

A Method of Direct Quantitation of Lactobacillus spp. in Intestinal Contents Based on Real-Time PCR.

Yu M Markova1, V V Stetsenko2, A S Polyanina2.   

Abstract

The method of direct quantitation of Lactobacillus spp. and L. acidophilus in intestinal contents based on real-time PCR was developed. It does not require culturing and allows estimating the number of living lactobacilli cells (measured in lg CFU) in absolute quantitative PCR format.
© 2021. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  gut microbiota; lactobacilli; real-time PCR

Mesh：

Substances：

Year:  2021        PMID： 34709511     DOI： 10.1007/s10517-021-05318-w

Source DB:  PubMed          Journal:  Bull Exp Biol Med        ISSN： 0007-4888            Impact factor:   0.804


  9 in total

1.  A PCR-based method for identification of lactobacilli at the genus level.

Authors:  Ségolène Dubernet; Nathalie Desmasures; Micheline Guéguen
Journal:  FEMS Microbiol Lett       Date:  2002-09-10       Impact factor: 2.742

2.  Identification method based on PCR combined with automated ribotyping for tracking probiotic Lactobacillus strains colonizing the human gut and vagina.

Authors:  M Massi; B Vitali; F Federici; D Matteuzzi; P Brigidi
Journal:  J Appl Microbiol       Date:  2004       Impact factor: 3.772

3.  Molecular methods to measure intestinal bacteria: a review.

Authors:  G Douglas Inglis; Matthew C Thomas; Dallas K Thomas; Martin L Kalmokoff; Stephen P J Brooks; L Brent Selinger
Journal:  J AOAC Int       Date:  2012 Jan-Feb       Impact factor: 1.913

Review 4.  Biodiversity of Intestinal Lactic Acid Bacteria in the Healthy Population.

Authors:  Marika Mikelsaar; Epp Sepp; Jelena Štšepetova; Epp Songisepp; Reet Mändar
Journal:  Adv Exp Med Biol       Date:  2016       Impact factor: 2.622

5.  The Genus Lactobacillus: A Taxonomic Update.

Authors:  Elisa Salvetti; Sandra Torriani; Giovanna E Felis
Journal:  Probiotics Antimicrob Proteins       Date:  2012-12       Impact factor: 4.609

6.  Development of oligonucleotide primers from the 16S-23S rRNA intergenic sequences for identifying different dairy and probiotic lactic acid bacteria by PCR.

Authors:  A Tilsala-Timisjärvi; T Alatossava
Journal:  Int J Food Microbiol       Date:  1997-03-18       Impact factor: 5.277

Review 7.  Intestinal Lactobacillus in health and disease, a driver or just along for the ride?

Authors:  Dustin D Heeney; Mélanie G Gareau; Maria L Marco
Journal:  Curr Opin Biotechnol       Date:  2017-09-01       Impact factor: 9.740

8.  Quantification of Bifidobacterium spp. and Lactobacillus spp. in rat fecal samples by real-time PCR.

Authors:  Jean-Marc Delroisse; Anne-Lise Boulvin; Isabelle Parmentier; Robin Dubois Dauphin; Micheline Vandenbol; Daniel Portetelle
Journal:  Microbiol Res       Date:  2008       Impact factor: 5.415

9.  Methanobrevibacter smithii is the predominant methanogen in patients with constipation-predominant IBS and methane on breath.

Authors:  Gene Kim; Fnu Deepinder; Walter Morales; Laura Hwang; Stacy Weitsman; Christopher Chang; Robert Gunsalus; Mark Pimentel
Journal:  Dig Dis Sci       Date:  2012-05-10       Impact factor: 3.199
  9 in total

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