Yuh-Feng Tsai1,2, Jai-Sing Yang3, Fuu-Jen Tsai4,5, Yih-Dih Cheng6,7, Yu-Jen Chiu8,9,10, Shih-Chang Tsai11. 1. Department of Diagnostic Radiology, Shin-Kong Wu Ho-Su Memorial Hospital, Taipei, Taiwan, R.O.C. 2. School of Medicine, Fu-Jen Catholic University, New Taipei, Taiwan, R.O.C. 3. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C. 4. School of Chinese Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan, R.O.C. 5. China Medical University Children's Hospital, China Medical University, Taichung, Taiwan, R.O.C. 6. School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C. 7. Department of Pharmacy, China Medical University Hospital, Taichung, Taiwan, R.O.C. 8. Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C.; chiou70202@gmail.com sctsai@mail.cmu.edu.tw. 9. Department of Surgery, School of Medicine, National Yang Ming University, Taipei, Taiwan, R.O.C. 10. Institute of Clinical Medicine, National Yang-Ming University, Taipei, Taiwan, R.O.C. 11. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. chiou70202@gmail.com sctsai@mail.cmu.edu.tw.
Abstract
BACKGROUND/AIM: The use of iodinated contrast media may impair renal function. However, no report has addressed the nephrotoxicity of high doses of iodinated contrast media in normal kidney cells and its associated molecular mechanisms. MATERIALS AND METHODS: Cell proliferation was assessed using the MTT assay. Cell death was evaluated through examining the morphological changes and TUNEL assay. Autophagy was detected through acridine orange staining and lysotracker staining. Reactive oxygen species production and AKT kinase activity were examined. RESULTS: Iopromide induced cell death and triggered apoptosis and autophagy in HEK 293 cells. Cell viability was significantly restored in the presence of a pan-caspase inhibitor or a ROS scavenger, N-acetyl-L-cysteine. AKT kinase activity was found to be reduced in iopromide-treated HEK 293 cells. CONCLUSION: High concentrations of iopromide induce cell damage, apoptosis, and autophagy through down-regulating AKT and ROS-activated cellular stress pathways in HEK 293 cells.
BACKGROUND/AIM: The use of iodinated contrast media may impair renal function. However, no report has addressed the nephrotoxicity of high doses of iodinated contrast media in normal kidney cells and its associated molecular mechanisms. MATERIALS AND METHODS: Cell proliferation was assessed using the MTT assay. Cell death was evaluated through examining the morphological changes and TUNEL assay. Autophagy was detected through acridine orange staining and lysotracker staining. Reactive oxygen species production and AKT kinase activity were examined. RESULTS: Iopromide induced cell death and triggered apoptosis and autophagy in HEK 293 cells. Cell viability was significantly restored in the presence of a pan-caspase inhibitor or a ROS scavenger, N-acetyl-L-cysteine. AKT kinase activity was found to be reduced in iopromide-treated HEK 293 cells. CONCLUSION: High concentrations of iopromide induce cell damage, apoptosis, and autophagy through down-regulating AKT and ROS-activated cellular stress pathways in HEK 293 cells.
Authors: Julian L Wichmann; Richard W Katzberg; Sheldon E Litwin; Peter L Zwerner; Carlo N De Cecco; Thomas J Vogl; Philip Costello; U Joseph Schoepf Journal: Circulation Date: 2015-11-17 Impact factor: 29.690
Authors: Shu Min Tao; Julian L Wichmann; U Joseph Schoepf; Stephen R Fuller; Guang Ming Lu; Long Jiang Zhang Journal: Eur Radiol Date: 2015-12-18 Impact factor: 5.315
Authors: I R Hutcheson; T M Griffith; M R Pitman; R Towart; M Gregersen; H Refsum; J O Karlsson Journal: Br J Pharmacol Date: 1999-09 Impact factor: 8.739