Yu-Jen Chiu1,2,3, Yu-Hsiang Lo4,5, Jai-Sing Yang5, Sheng-Chu Kuo6, Shih-Chang Tsai7. 1. Division of Plastic and Reconstructive Surgery, Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.C. 2. Department of Surgery, School of Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C. 3. Institute of Clinical Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan, R.O.C. 4. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C. 5. Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan, R.O.C. 6. Chinese Medicinal Research and Development Center, China Medical University Hospital, School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C. 7. Department of Biological Science and Technology, China Medical University, Taichung, Taiwan, R.O.C.; sctsai@mail.cmu.edu.tw.
Abstract
BACKGROUND/AIM: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C2C12 cells. MATERIALS AND METHODS: Cell viability was examined using the MTT assay. C2C12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay. RESULTS: Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in C2C12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. CONCLUSION: MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.
BACKGROUND/AIM: At present, there are no effective drugs for the treatment of insulin resistance. MTH-3, a curcumin derivative, exerts potent anti-cancer effects. The aim of the present study was to explore whether MTH-3 is capable of regulating palmitic acid (PA)-induced insulin resistance in C2C12 cells. MATERIALS AND METHODS: Cell viability was examined using the MTT assay. C2C12 cells were treated with PA and evaluated for the production of oil droplets using an Oil Red O assay. Glucose uptake was analysed by the 2-NBDG assay. RESULTS: Treatment of cells with up to 500 μM PA for 24 h or with 5 or 10 μM MTH-3 had no effect on cell viability. PA induced production of oil droplets in C2C12 cells. After adding MTH-3, the quantity of oil droplets decreased significantly and glucose uptake recovered. CONCLUSION: MTH-3 may become an efficient drug for the treatment of insulin resistance and associated diseases.
Authors: Kathryn A Junkin; David J Dyck; Kerry L Mullen; Adrian Chabowski; A Brianne Thrush Journal: Am J Physiol Regul Integr Comp Physiol Date: 2009-02-04 Impact factor: 3.619