Yusuke Aoki1,2,3, Jun Yamamoto1,2,4, Yasunori Tome5, Kazuyuki Hamada1,2, Noriyuki Masaki1,2, Sachiko Inubushi6, Yoshihiko Tashiro1, Michael Bouvet2, Itaru Endo4, Kotaro Nishida3, Robert M Hoffman7,2. 1. AntiCancer Inc, San Diego, CA, U.S.A. 2. Department of Surgery, University of California San Diego, La Jolla, CA, U.S.A. 3. Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan. 4. Department of Gastroenterological Surgery, Yokohama City University Graduate School of Medicine, Yokohama, Japan. 5. Department of Orthopedic Surgery, Graduate School of Medicine, University of the Ryukyus, Nishihara, Japan; yastome@med.u-ryukyu.ac.jp. 6. Department of Breast and Endocrine Surgery, Kobe University Graduate School of Medicine, Hyogo, Japan. 7. AntiCancer Inc, San Diego, CA, U.S.A.; all@anticancer.com.
Abstract
BACKGROUND/AIM: Sarcomas are considered a heterogeneous disease with incomplete understanding of its molecular basis. In the present study, to further understand general molecular changes in sarcoma, patient-derived xenograft (PDX) mouse models of the three most common soft-tissue sarcomas: myxofibrosarcoma, undifferentiated pleomorphic sarcoma (UPS) and liposarcoma were established and the methylation status of histone H3 lysine marks was studied. MATERIALS AND METHODS: Immunoblotting and immunohistochemical staining were used to quantify the extent of methylation of histone H3K4me3 and histone H3K9me3. RESULTS: In all 3 sarcoma types in PDX models, histone H3K4me3 and H3K9me3 were found highly over-methylated compared to normal muscle tissue. CONCLUSION: Histone H3 lysine over-methylation may be a general basis of malignancy of the major sarcoma types. Copyright
BACKGROUND/AIM: Sarcomas are considered a heterogeneous disease with incomplete understanding of its molecular basis. In the present study, to further understand general molecular changes in sarcoma, patient-derived xenograft (PDX) mouse models of the three most common soft-tissue sarcomas: myxofibrosarcoma, undifferentiated pleomorphic sarcoma (UPS) and liposarcoma were established and the methylation status of histone H3 lysine marks was studied. MATERIALS AND METHODS: Immunoblotting and immunohistochemical staining were used to quantify the extent of methylation of histone H3K4me3 and histone H3K9me3. RESULTS: In all 3 sarcoma types in PDX models, histone H3K4me3 and H3K9me3 were found highly over-methylated compared to normal muscle tissue. CONCLUSION: Histone H3 lysine over-methylation may be a general basis of malignancy of the major sarcoma types. Copyright
Authors: Kentaro Igarashi; Kei Kawaguchi; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyaki; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Takashi Higuchi; Arun S Singh; Bartosz Chmielowski; Scott D Nelson; Tara A Russell; Mark A Eckardt; Sarah M Dry; Yunfeng Li; Shree Ram Singh; Sant P Chawla; Fritz C Eilber; Hiroyuki Tsuchiya; Robert M Hoffman Journal: Biochem Biophys Res Commun Date: 2018-11-02 Impact factor: 3.575
Authors: Kentaro Igarashi; Kei Kawaguchi; Shukuan Li; Qinghong Han; Yuying Tan; Takashi Murakami; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Arun S Singh; Mark A Eckardt; Scott D Nelson; Tara A Russell; Sarah M Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Shree Ram Singh; Fritz C Eilber; Robert M Hoffman Journal: Cancer Lett Date: 2018-01-03 Impact factor: 8.679
Authors: Tasuku Kiyuna; Yasunori Tome; Takashi Murakami; Kentaro Miyake; Kentaro Igarashi; Kei Kawaguchi; Hiromichi Oshiro; Takashi Higuchi; Masuyo Miyake; Norihiko Sugisawa; Zhiying Zhang; Sahar Razmjooei; Sintawat Wangsiricharoen; Bartosz Chmielowski; Scott D Nelson; Tara A Russell; Sarah M Dry; Yunfeng Li; Mark A Eckardt; Arun S Singh; Sant Chawla; Fuminori Kanaya; Fritz C Eilber; Shree Ram Singh; Ming Zhao; Robert M Hoffman Journal: Biochem Biophys Res Commun Date: 2018-10-03 Impact factor: 3.575
Authors: Kentaro Igarashi; Kei Kawaguchi; Shukuan Li; Qinghong Han; Yuying Tan; Emily Gainor; Tasuku Kiyuna; Kentaro Miyake; Masuyo Miyake; Takashi Higuchi; Hiromichi Oshiro; Arun S Singh; Mark A Eckardt; Scott D Nelson; Tara A Russell; Sarah M Dry; Yunfeng Li; Norio Yamamoto; Katsuhiro Hayashi; Hiroaki Kimura; Shinji Miwa; Hiroyuki Tsuchiya; Fritz C Eilber; Robert M Hoffman Journal: Oncotarget Date: 2018-04-10