Hadi Ghaffari1, Ahmad Tavakoli2, Mohammad Faranoush3, Ali Naderi4, Seyed Jalal Kiani5, Alireza Sadeghipour6, Davod Javanmard7, Mohammad Farahmand8, Saied Ghorbani5, Farnoush Sedaghati9, Seyed Hamidreza Monavari5. 1. Department of Bacteriology and Virology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran. 2. Research Center of Pediatric Infectious Diseases, Institute of Immunology and Infectious Diseases, Iran University of Medical Sciences, Tehran, Iran. 3. Pediatric Growth and Development Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences, Tehran, Iran. 4. Mahak Pediatric Cancer Treatment and Research Center, Tehran, Iran. 5. Department of Medical Virology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran. 6. Department of Pathology, Hazrat-e-Rasoul Akram Hospital, Iran University of Medical Sciences, Tehran, Iran. 7. Infectious Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran. 8. Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. 9. Department of Pathology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran.
Abstract
Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) infections in patients with glioblastoma multiforme (GBM). Methods: This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results: HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion: Our findings indicate that infection with EBV is associated with GBM.
Background: Glioblastoma multiforme is the most invasive and lethal form of brain cancer with unclear etiology. Our study aimed to investigate the molecular prevalence of human cytomegalovirus (HCMV) and Epstein-Barr virus (EBV) infections in patients with glioblastoma multiforme (GBM). Methods: This case-control study was conducted on 42 FFPE brain tumor samples from GBM patients and 42 brain autopsies from subjects without neurological disorders. The presence of EBV and HCMV DNA was determined, using PCR and nested-PCR assays, respectively. Results: HCMV DNA was detected in 3 out of 42 (7.1%) of GBM samples and was absent from the control group (p = 0.07). Importantly, EBV DNA was detected in 9 out of 42 (21.4%) brain tissue specimens of GBM subjects, but again in none of the control group (p = 0.001). Conclusion: Our findings indicate that infection with EBV is associated with GBM.
Entities:
Keywords:
Brain tumor; Epstein-Barr virus; Glioblastoma; Human cytomegalovirus
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