Literature DB >> 3469617

Corticosteroid metabolism in rat kidney in vitro. IV. Subcellular, sites of 11 beta-hydroxysteroid dehydrogenase activity.

N Kobayashi, W Schulz, K Hierholzer.   

Abstract

An attempt has been made to identify the subcellular localization of renal corticosteroid metabolism. Subcellular fractions were prepared by differential centrifugation, identified by marker enzymes and incubated under different conditions with corticosterone (B). The NADP+/NADPH dependent metabolism of B could be localized in the nuclear and microsomal fraction. The most prominent metabolite was 11-dehydro-B, which is formed by 11 beta-hydroxysteroid dehydrogenase (EC 1.1.1.146). Enzyme kinetic studies of this enzyme with B as substrate revealed apparent Km-values in the range of 10(-7) M for both the nuclear and microsomal fraction.

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Year:  1987        PMID： 3469617     DOI： 10.1007/BF00581839

Source DB:  PubMed          Journal:  Pflugers Arch        ISSN： 0031-6768            Impact factor:   3.657


  25 in total

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Journal:  Nature       Date:  1965-02-20       Impact factor: 49.962

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Journal:  Biochim Biophys Acta       Date:  1970-12-08

5.  Separation of the hormone- and DNA-binding sites of the hepatic glucocorticoid receptor by means of proteolysis.

Authors:  O Wrange; J A Gustafsson
Journal:  J Biol Chem       Date:  1978-02-10       Impact factor: 5.157

6.  25-Hydroxycholecalciferol-1-hydroxylase: a specific requirement for NADPH and a hemoprotein component in chick kidney mitochondria.

Authors:  J G Ghazarian; H F DeLuca
Journal:  Arch Biochem Biophys       Date:  1974-01       Impact factor: 4.013

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Authors:  T Mishina; D W Scholer; I S Edelman
Journal:  Am J Physiol       Date:  1981-01

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Authors:  C A Winkel; E R Simpson; L Milewich; P C MacDonald
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

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Authors:  S M Lee; M A Chekal; A I Katz
Journal:  Am J Physiol       Date:  1983-05

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Authors:  S W Golf; F Bepperling; V Graef
Journal:  Steroids       Date:  1984-01       Impact factor: 2.668
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  4 in total

1.  Distribution of 11 beta-hydroxysteroid dehydrogenase along the rabbit nephron.

Authors:  J P Bonvalet; I Doignon; M Blot-Chabaud; P Pradelles; N Farman
Journal:  J Clin Invest       Date:  1990-09       Impact factor: 14.808

2.  Bile acids and their amidates inhibit 11 beta-hydroxysteroid dehydrogenase obtained from rat kidney.

Authors:  F H Perschel; H Bühler; K Hierholzer
Journal:  Pflugers Arch       Date:  1991-07       Impact factor: 3.657

3.  Contraluminal p-aminohippurate transport in the proximal tubule of the rat kidney. VIII. Transport of corticosteroids.

Authors:  K J Ullrich; G Rumrich; F Papavassiliou; K Hierholzer
Journal:  Pflugers Arch       Date:  1991-05       Impact factor: 3.657

4.  Aldosterone metabolism in rat renal tissue in vitro. Formation of lipid soluble metabolites.

Authors:  D l'Allemand; H Siebe; D Tsiakiras; G A Hoyer; P Vecsei; K Hierholzer
Journal:  Pflugers Arch       Date:  1988-05       Impact factor: 3.657
  4 in total

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