Takaomi Hagi1, Yuji Ishii2, Kotaro Yamashita1, Takuro Saito1, Koji Tanaka1, Tomoki Makino1, Tsuyoshi Takahashi1, Yukinori Kurokawa1, Makoto Yamasaki1, Hidetoshi Eguchi1, Yuichiro Doki1, Kiyokazu Nakajima3,4. 1. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan. 2. CASTEM Co., Ltd., Fukuyama, Japan. 3. Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Osaka, Japan. knakajima@gesurg.med.osaka-u.ac.jp. 4. Department of Next Generation Endoscopic Intervention (Project ENGINE), Center of Medical Innovation and Translational Research, Osaka University Graduate School of Medicine, Suite 0912, 2‑2, Yamadaoka, Suita, Osaka, 565‑0871, Japan. knakajima@gesurg.med.osaka-u.ac.jp.
Abstract
PURPOSE: Microfocus computed tomography (micro-CT) has not been widely used at high radiation intensity (industrial micro-CT) in life science fields. In this preliminary study, we investigated its potential value in the detection of micro-hepatic tumors in a mouse model. METHODS: The liver with micro-hepatic tumors was surgically resected en-bloc from mice, and examined with industrial micro-CT and lower intensity micro-CT (small animal micro-CT). The number of hepatic tumors was manually counted on serial images. Then, the accuracy of each technique was determined by preparing matching liver sections and comparing the number of tumors identified in a conventional pathological examination. RESULTS: The number of hepatic tumors evaluated with industrial micro-CT showed high concordance with the results of the pathological examinations (intraclass correlation coefficient [ICC]: 0.984; 95% confidence interval [CI] 0.959-0.994). On the other hand, the number of hepatic tumors evaluated with the small animal micro-CT showed low concordance with the number identified in the pathological examinations (ICC: 0.533; 95% CI 0.181-0.815). CONCLUSION: Industrial micro-CT improved the detection of small structures in resected specimens, and might be a promising solution for life science research.
PURPOSE: Microfocus computed tomography (micro-CT) has not been widely used at high radiation intensity (industrial micro-CT) in life science fields. In this preliminary study, we investigated its potential value in the detection of micro-hepatic tumors in a mouse model. METHODS: The liver with micro-hepatic tumors was surgically resected en-bloc from mice, and examined with industrial micro-CT and lower intensity micro-CT (small animal micro-CT). The number of hepatic tumors was manually counted on serial images. Then, the accuracy of each technique was determined by preparing matching liver sections and comparing the number of tumors identified in a conventional pathological examination. RESULTS: The number of hepatic tumors evaluated with industrial micro-CT showed high concordance with the results of the pathological examinations (intraclass correlation coefficient [ICC]: 0.984; 95% confidence interval [CI] 0.959-0.994). On the other hand, the number of hepatic tumors evaluated with the small animal micro-CT showed low concordance with the number identified in the pathological examinations (ICC: 0.533; 95% CI 0.181-0.815). CONCLUSION: Industrial micro-CT improved the detection of small structures in resected specimens, and might be a promising solution for life science research.
Authors: Rong Tang; Suzanne B Coopey; Julliette M Buckley; Owen P Aftreth; Leopoldo J Fernandez; Elena F Brachtel; James S Michaelson; Michele A Gadd; Michelle C Specht; Frederick C Koerner; Barbara L Smith Journal: Breast J Date: 2013-06-17 Impact factor: 2.431
Authors: Jan Marie de Gooyer; Fortuné M K Elekonawo; Desirée L Bos; Rachel S van der Post; André Pèlegrin; Bérénice Framery; Françoise Cailler; Alexander L Vahrmeijer; Johannes H W de Wilt; Mark Rijpkema Journal: Clin Cancer Res Date: 2020-09-08 Impact factor: 12.531