Literature DB >> 34674178

Firefly Luciferase Complementation-Based Analysis of Dynamic Protein-Protein Interactions Under Diurnal and Circadian Conditions in Arabidopsis.

Qiguang Xie1,2, Qiao Wang3, Xiaodong Xu3,4.   

Abstract

Split firefly luciferase complementation assay (FLCA) is one of the most widely used sensitive and reliable methods for the analysis of constitutive and dynamic protein-protein interactions (PPIs). Here, we report a method for long-term in vivo detects plant protein-protein interactions in Arabidopsis F1 hybrids via Topcount™ Microplate Scintillation Counter or Deep-Cooled CCD camera. Following these protocols, we successfully detected time-dependent PPIs of EARLY FLOWERING 3 (ELF3) and EARLY FLOWERING 4 (ELF4); both of them with LUX ARRHYTHMO (LUX) belong to an evening complex which has been found to play a key role in circadian rhythms, flowering, and growth.
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Bioluminescence; Circadian rhythm; Diurnal rhythm; Dynamic protein-protein interactions; Firefly luciferase complementation

Mesh：

Substances：

Year:  2022        PMID： 34674178     DOI： 10.1007/978-1-0716-1912-4_16

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  15 in total

Review 1.  Integrating circadian dynamics with physiological processes in plants.

Authors:  Kathleen Greenham; C Robertson McClung
Journal:  Nat Rev Genet       Date:  2015-09-15       Impact factor: 53.242

2.  NanoLuc Complementation Reporter Optimized for Accurate Measurement of Protein Interactions in Cells.

Authors:  Andrew S Dixon; Marie K Schwinn; Mary P Hall; Kris Zimmerman; Paul Otto; Thomas H Lubben; Braeden L Butler; Brock F Binkowski; Thomas Machleidt; Thomas A Kirkland; Monika G Wood; Christopher T Eggers; Lance P Encell; Keith V Wood
Journal:  ACS Chem Biol       Date:  2015-12-10       Impact factor: 5.100

3.  A highly sensitive protein-protein interaction assay based on Gaussia luciferase.

Authors:  Ingrid Remy; Stephen W Michnick
Journal:  Nat Methods       Date:  2006-11-12       Impact factor: 28.547

4.  Bioluminescent indicator for determining protein-protein interactions using intramolecular complementation of split click beetle luciferase.

Authors:  Sung Bae Kim; Yosuke Otani; Yoshio Umezawa; Hiroaki Tao
Journal:  Anal Chem       Date:  2007-06-01       Impact factor: 6.986

Review 5.  Molecular mechanisms at the core of the plant circadian oscillator.

Authors:  Maria A Nohales; Steve A Kay
Journal:  Nat Struct Mol Biol       Date:  2016-12-06       Impact factor: 15.369

6.  Luciferase Complementation Assay for Protein-Protein Interactions in Plants.

Authors:  Zhaoyang Zhou; Guozhi Bi; Jian-Min Zhou
Journal:  Curr Protoc Plant Biol       Date:  2018-03

7.  Anodontia as the sole clinical sign of the ectrodactyly-ectodermal dysplasia-cleft lip (EEC) syndrome.

Authors:  K H Chranowska; M Krajewska-Walasek; Z Rump; L Wisniewski; J P Fryns
Journal:  Genet Couns       Date:  1990

8.  High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase.

Authors:  Sung Bae Kim; Takeaki Ozawa; Shigeaki Watanabe; Yoshio Umezawa
Journal:  Proc Natl Acad Sci U S A       Date:  2004-08-02       Impact factor: 11.205

Review 9.  Surveying the landscape of optogenetic methods for detection of protein-protein interactions.

Authors:  Matthew D Wiens; Robert E Campbell
Journal:  Wiley Interdiscip Rev Syst Biol Med       Date:  2018-01-15

Review 10.  Protein-protein interaction detection: methods and analysis.

Authors:  V Srinivasa Rao; K Srinivas; G N Sujini; G N Sunand Kumar
Journal:  Int J Proteomics       Date:  2014-02-17
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.