Chandraprakash Yamini1, Govindasamy Sharmila2, Chandrasekaran Muthukumaran2, Kumar Pavithran1, Narasimhan Manojkumar3. 1. Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamilnadu, 603203, India. 2. Department of Industrial Biotechnology, Government College of Technology, Coimbatore, Tamilnadu, 641013, India. 3. Department of Genetic Engineering, School of Bioengineering, SRM Institute of Science and Technology, Kattankulathur, Chennai, Tamilnadu, 603203, India. manojkun@srmist.edu.in.
Abstract
INTRODUCTION: Thermotolerant microbes are a group of microorganisms that survive in elevated temperatures. The thermotolerant microbes, which are found in geothermal heat zones, grow at temperatures of or above 45°C. The proteins present in such microbes are optimally active at these elevated temperatures. Hence, therefore, serves as an advantage in various biotechnological applications. In the last few years, scientists have tried to understand the molecular mechanisms behind the maintenance of the structural integrity of the cell and to study the stability of various thermotolerant proteins at extreme temperatures. Proteomic analysis is the solution for this search. Applying novel proteomic tools determines the proteins involved in the thermostability of microbes at elevated temperatures. METHODS: Advanced proteomic techniques like Mass spectrometry, nano-LC-MS, protein microarray, ICAT, iTRAQ, and SILAC could enable the screening and identification of novel thermostable proteins. RESULTS: This review provides up-to-date details on the protein signature of various thermotolerant microbes analyzed through advanced proteomic tools concerning relevant research articles. The protein complex composition from various thermotolerant microbes cultured at different temperatures, their structural arrangement, and functional efficiency of the protein was reviewed and reported. CONCLUSION: This review provides an overview of thermotolerant microbes, their enzymes, and the proteomic tools implemented to characterize them. This article also reviewed a comprehensive view of the current proteomic approaches for protein profiling in thermotolerant microbes.
INTRODUCTION: Thermotolerant microbes are a group of microorganisms that survive in elevated temperatures. The thermotolerant microbes, which are found in geothermal heat zones, grow at temperatures of or above 45°C. The proteins present in such microbes are optimally active at these elevated temperatures. Hence, therefore, serves as an advantage in various biotechnological applications. In the last few years, scientists have tried to understand the molecular mechanisms behind the maintenance of the structural integrity of the cell and to study the stability of various thermotolerant proteins at extreme temperatures. Proteomic analysis is the solution for this search. Applying novel proteomic tools determines the proteins involved in the thermostability of microbes at elevated temperatures. METHODS: Advanced proteomic techniques like Mass spectrometry, nano-LC-MS, protein microarray, ICAT, iTRAQ, and SILAC could enable the screening and identification of novel thermostable proteins. RESULTS: This review provides up-to-date details on the protein signature of various thermotolerant microbes analyzed through advanced proteomic tools concerning relevant research articles. The protein complex composition from various thermotolerant microbes cultured at different temperatures, their structural arrangement, and functional efficiency of the protein was reviewed and reported. CONCLUSION: This review provides an overview of thermotolerant microbes, their enzymes, and the proteomic tools implemented to characterize them. This article also reviewed a comprehensive view of the current proteomic approaches for protein profiling in thermotolerant microbes.
Authors: Katrina Sandona; Terri L Billingsley Tobias; Miriam I Hutchinson; Donald O Natvig; Andrea Porras-Alfaro Journal: Mycologia Date: 2019-07-26 Impact factor: 2.696
Authors: An Coorevits; Valerie De Jonghe; Joachim Vandroemme; Rieka Reekmans; Jeroen Heyrman; Winy Messens; Paul De Vos; Marc Heyndrickx Journal: Syst Appl Microbiol Date: 2008-04-10 Impact factor: 4.022
Authors: Susana Delgado; Caio T C C Rachid; Elena Fernández; Tomasz Rychlik; Angel Alegría; Raquel S Peixoto; Baltasar Mayo Journal: Food Microbiol Date: 2013-05-06 Impact factor: 5.516