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Literature DB >> 3467155

Transformation of the basidiomycete, Schizophyllum commune.

A Munoz-Rivas, C A Specht, B J Drummond, E Froeliger, C P Novotny, R C Ullrich.

Abstract

Protoplasts of a Schizophyllum commune tryptophan auxotroph (trp1), deficient in indole-3-glycerol phosphate synthetase (IGPS), were transformed to trp+ with plasmid DNA containing the Schizophyllum TRP1 sequence. Efficiencies up to 30 transformants per microgram of plasmid DNA were obtained. Southern blots reveal that the transforming DNA is integrated in chromosomal DNA. The trp+ phenotype of transformants is stable in meiosis and mitosis. Transformants possess IGPS activity comparable to wild-type cells.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA, Fungal

Year: 1986 PMID： 3467155 DOI： 10.1007/BF02428038

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

10 in total

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Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. High-frequency transformation of the fission yeast Schizosaccharomyces pombe.

Authors: D Beach; P Nurse
Journal: Nature Date: 1981-03-12 Impact factor: 49.962

3. Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors: D B Clewell
Journal: J Bacteriol Date: 1972-05 Impact factor: 3.490

4. A method for extracting high-molecular-weight deoxyribonucleic acid from fungi.

Authors: C A Specht; C C DiRusso; C P Novotny; R C Ullrich
Journal: Anal Biochem Date: 1982-01-01 Impact factor: 3.365

5. Transformation of Kluyveromyces lactis with the kanamycin (G418) resistance gene of Tn903.

Authors: K Sreekrishna; T D Webster; R C Dickson
Journal: Gene Date: 1984-04 Impact factor: 3.688

6. Transformation of Kluyveromyces fragilis.

Authors: S Das; E Kellermann; C P Hollenberg
Journal: J Bacteriol Date: 1984-06 Impact factor: 3.490

7. Transformation of yeast by a replicating hybrid plasmid.

Authors: J D Beggs
Journal: Nature Date: 1978-09-14 Impact factor: 49.962

8. Transformation of yeast.

Authors: A Hinnen; J B Hicks; G R Fink
Journal: Proc Natl Acad Sci U S A Date: 1978-04 Impact factor: 11.205

9. Structure of the trifunctional trp-1 gene from Neurospora crassa and its aberrant expression in Escherichia coli.

Authors: M G Schechtman; C Yanofsky
Journal: J Mol Appl Genet Date: 1983

10. Isolation of the DNA sequence coding indole-3-glycerol phosphate synthetase and phosphoribosylanthranilate isomerase of Schizophyllum commune.

Authors: A M Muñoz-Rivas; C A Specht; R C Ullrich; C P Novotny
Journal: Curr Genet Date: 1986 Impact factor: 3.886

10 in total

19 in total

1. Transformation by Complementation of an Adenine Auxotroph of the Lignin-Degrading Basidiomycete Phanerochaete chrysosporium.

Authors: M Alic; J R Kornegay; D Pribnow; M H Gold
Journal: Appl Environ Microbiol Date: 1989-02 Impact factor: 4.792

5. Transformation as a method of increasing gene copy number and gene expression in the basidiomycete fungus Coprinus cinereus.

Authors: F M Mellon; L A Casselton
Journal: Curr Genet Date: 1988-11 Impact factor: 3.886

9. Homologous transformation of the edible basidiomycete Agrocybe aegerita with the URA1 gene: characterization of integrative events and of rearranged free plasmids in transformants.

Authors: T Noël; J Labarère
Journal: Curr Genet Date: 1994-05 Impact factor: 3.886

10. A mushroom-inducing DNA sequence isolated from the Basidiomycete, Schizophyllum commune.

Authors: J S Horton; C A Raper
Journal: Genetics Date: 1991-11 Impact factor: 4.562