Lina Liu1, Luran Liu1, Yunting Lu1, Tianyuan Zhang1, Wenting Zhao1. 1. Department of Neurology, Science & Technology Innovation Park of The Fourth Affiliated Hospital of Harbin Medical University, Harbin City, Heilongjiang Province, 150028, China.
Abstract
Aim: This study aimed to evaluate the effect of miR-24-3p in Alzheimer's disease (AD). Materials & methods: A total of 198 participants were recruited in this study, including 104 AD patients and 94 healthy controls. Expression of miR-24-3p was detected using quantitative real-time PCR. Receiver-operating characteristic curve was used to assess the diagnostic value of miR-24-3p. In vitro AD model was established to evaluate the effect of miR-24-3p. The downstream target was detected by luciferase reporter gene assay. Results: Expression of miR-24-3p showed 1.6-fold increase in AD group compared with healthy controls, and a negative correlation of miR-24-3p with mini-mental state examination score was obtained. Receiver-operating characteristic curve showed satisfactory diagnostic accuracy. Downregulation of miR-24-3p promoted cell proliferation and inhibited cell apoptosis. KLF8 is a target gene of miR-24-3p. Conclusion: MiR-24-3p has a certain value in the diagnosis of AD and may be a potential biomarker.
Aim: This study aimed to evaluate the effect of miR-24-3p in Alzheimer's disease (AD). Materials & methods: A total of 198 participants were recruited in this study, including 104 AD patients and 94 healthy controls. Expression of miR-24-3p was detected using quantitative real-time PCR. Receiver-operating characteristic curve was used to assess the diagnostic value of miR-24-3p. In vitro AD model was established to evaluate the effect of miR-24-3p. The downstream target was detected by luciferase reporter gene assay. Results: Expression of miR-24-3p showed 1.6-fold increase in AD group compared with healthy controls, and a negative correlation of miR-24-3p with mini-mental state examination score was obtained. Receiver-operating characteristic curve showed satisfactory diagnostic accuracy. Downregulation of miR-24-3p promoted cell proliferation and inhibited cell apoptosis. KLF8 is a target gene of miR-24-3p. Conclusion: MiR-24-3p has a certain value in the diagnosis of AD and may be a potential biomarker.
Authors: Maria Serpente; Laura Ghezzi; Chiara Fenoglio; Francesca R Buccellato; Giorgio G Fumagalli; Emanuela Rotondo; Marina Arcaro; Andrea Arighi; Daniela Galimberti Journal: Int J Mol Sci Date: 2022-07-30 Impact factor: 6.208