Literature DB >> 3466805

Detection of the major pertussis toxin substrate of human leukocytes with antisera raised against synthetic peptides.

J Falloon, H Malech, G Milligan, C Unson, R Kahn, P Goldsmith, A Spiegel.   

Abstract

Antisera raised against the carboxy-terminal decapeptide (KENLKDCGLF) of transducin-alpha detected the 40 kDa, major pertussis toxin substrate of human neutrophils. The antisera also detected this protein in undifferentiated HL-60 and U937 cells, and revealed an approx. 2-fold increase in protein/mg membrane protein with differentiation into mature phagocytic cells. The results provide direct immunochemical evidence for the presence of a novel, pertussis toxin-sensitive guanine nucleotide-binding protein in human leukocytes.

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Year:  1986        PMID： 3466805     DOI： 10.1016/0014-5793(86)81141-3

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  15 in total

1.  Subcellular distribution of the alpha subunit(s) of Gi: visualization by immunofluorescent and immunogold labeling.

Authors:  J M Lewis; M J Woolkalis; G L Gerton; R M Smith; L Jarett; D R Manning
Journal:  Cell Regul       Date:  1991-12

2.  Differential G-protein expression during B- and T-cell development.

Authors:  K R Grant; W Harnett; G Milligan; M M Harnett
Journal:  Immunology       Date:  1997-04       Impact factor: 7.397

3.  The effect of cholera toxin on the inhibition of vasopressin-stimulated inositol phospholipid hydrolysis is a cyclic AMP-mediated event at the level of receptor binding.

Authors:  S D Gardner; G Milligan; J E Rice; M J Wakelam
Journal:  Biochem J       Date:  1989-05-01       Impact factor: 3.857

Review 4.  Rous-Whipple award lecture. The formylpeptide receptor of the neutrophil. A search and conserve operation.

Authors:  E L Becker
Journal:  Am J Pathol       Date:  1987-10       Impact factor: 4.307

Review 5.  Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins.

Authors:  G Milligan
Journal:  Biochem J       Date:  1988-10-01       Impact factor: 3.857

6.  Foetal-calf serum stimulates a pertussis-toxin-sensitive high-affinity GTPase activity in rat glioma C6 BU1 cells.

Authors:  G Milligan
Journal:  Biochem J       Date:  1987-07-15       Impact factor: 3.857

7.  Activating and inactivating mutations of the alpha subunit of Gi2 protein have opposite effects on proliferation of NIH 3T3 cells.

Authors:  S Hermouet; J J Merendino; J S Gutkind; A M Spiegel
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-01       Impact factor: 11.205

8.  Antibodies which recognize the C-terminus of the inhibitory guanine-nucleotide-binding protein (Gi) demonstrate that opioid peptides and foetal-calf serum stimulate the high-affinity GTPase activity of two separate pertussis-toxin substrates.

Authors:  F R McKenzie; E C Kelly; C G Unson; A M Spiegel; G Milligan
Journal:  Biochem J       Date:  1988-02-01       Impact factor: 3.857

Review 9.  The structure and mechanism of neurotransmitter receptors. Implications for the structure and function of the central nervous system.

Authors:  P G Strange
Journal:  Biochem J       Date:  1988-01-15       Impact factor: 3.857

10.  Relationship between arachidonate release and exocytosis in permeabilized human neutrophils stimulated with formylmethionyl-leucyl-phenylalanine (fMetLeuPhe), guanosine 5'-[gamma-thio]triphosphate (GTP[S]) and Ca2+.

Authors:  S Cockcroft
Journal:  Biochem J       Date:  1991-04-01       Impact factor: 3.857
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