Ozlem Sezer1, Canan Soyer Çalışkan2, Samettin Celik2, Suleyman Sirri Kilic3, Tuba Kuruoglu4, Goksenin Unluguzel Ustun5, Nazan Yurtcu6. 1. Department of Medical Genetics, Samsun Education and Research Hospital, University of Health Sciences, Samsun, Turkey. 2. Department of Obstetrics and Gynecology, Samsun Education and Research Hospital, University of Health Sciences, Samsun, Turkey. 3. Department of Infectious Diseases and Clinical Microbiology, Samsun Education and Research Hospital, University of Health Sciences, Samsun, Turkey. 4. Department of Clinical Microbiology and Infectious Diseases, Ondokuz Mayıs University Faculty of Medicine, Samsun, Turkey. 5. Department of Biochemistry, Samsun Education and Research Hospital, University of Health Sciences, Samsun, Turkey. 6. Department of Obstetrics and Gynecology, Sivas Cumhuriyet University Faculty of Medicine, Sivas, Turkey.
Abstract
AIM: Microbiota of the reproductive tract may be associated with unexplained infertility in women. We aimed to determine the derangements of vaginal and endometrial microbiota related to unexplained infertility by real-time polymerase chain reaction (real-time PCR) microbiota analyses of vaginal and endometrial samples of the unexplained infertility patients and fertile women. MATERIALS AND METHODS: Twenty-six women with unexplained infertility and 26 age-matched fertile women were included. Vaginal and endometrial samples were obtained in the mid-menstrual cycle for analysis by quantitative real-time PCR method. RESULTS: The proportion of lactobacilli-impaired microbiota was significantly higher in the vaginal samples of unexplained infertility patients (76.9% vs. 26.9%; p < 0.001). Those with impaired lactobacilli microbiota of vaginal samples had an increased risk of 9.048 times for infertility than those with normal lactobacilli microbiota. In addition, the mean lactobacilli/total bacterial mass (TBM) ratio in the vaginal samples was significantly lower in the unexplained infertility patients (38.2% vs.76.3%; p = 0.001). CONCLUSION: The present study results supported the role of vaginal and endometrial microbiota derangements in unexplained infertility. Many time-consuming and invasive methods are currently used in the diagnosis of infertility. Our study showed that the quantitative determination of lactobacilli/TBM ratio in vaginal specimens, a less invasive and easily obtainable method, could be used as a diagnostic test during the workup of couples with unexplained infertility.
AIM: Microbiota of the reproductive tract may be associated with unexplained infertility in women. We aimed to determine the derangements of vaginal and endometrial microbiota related to unexplained infertility by real-time polymerase chain reaction (real-time PCR) microbiota analyses of vaginal and endometrial samples of the unexplained infertility patients and fertile women. MATERIALS AND METHODS: Twenty-six women with unexplained infertility and 26 age-matched fertile women were included. Vaginal and endometrial samples were obtained in the mid-menstrual cycle for analysis by quantitative real-time PCR method. RESULTS: The proportion of lactobacilli-impaired microbiota was significantly higher in the vaginal samples of unexplained infertility patients (76.9% vs. 26.9%; p < 0.001). Those with impaired lactobacilli microbiota of vaginal samples had an increased risk of 9.048 times for infertility than those with normal lactobacilli microbiota. In addition, the mean lactobacilli/total bacterial mass (TBM) ratio in the vaginal samples was significantly lower in the unexplained infertility patients (38.2% vs.76.3%; p = 0.001). CONCLUSION: The present study results supported the role of vaginal and endometrial microbiota derangements in unexplained infertility. Many time-consuming and invasive methods are currently used in the diagnosis of infertility. Our study showed that the quantitative determination of lactobacilli/TBM ratio in vaginal specimens, a less invasive and easily obtainable method, could be used as a diagnostic test during the workup of couples with unexplained infertility.
Authors: Lela K Keburiya; Veronika Yu Smolnikova; Tatiana V Priputnevich; Vera V Muravieva; Alexey B Gordeev; Dmitry Yu Trofimov; Ekaterina S Shubina; Taisiya O Kochetkova; Margarita S Rogacheva; Elena A Kalinina; Gennady T Sukhikh Journal: BMC Womens Health Date: 2022-05-14 Impact factor: 2.742
Authors: Anna Bednarska-Czerwińska; Michał Czerwiński; Emilia Morawiec; Aleksandra Łach; Anna Ziaja; Adrian Kusaj; Patrycja Strączyńska; Dorota Sagan; Dariusz Boroń; Beniamin Oskar Grabarek Journal: J Clin Med Date: 2022-06-10 Impact factor: 4.964