| Literature DB >> 34648371 |
Inna Ricardo-Lax1, Joseph M Luna1, Tran Thi Nhu Thao2,3,4, Jérémie Le Pen1, Yingpu Yu1, H-Heinrich Hoffmann1, William M Schneider1, Brandon S Razooky1, Javier Fernandez-Martinez5, Fabian Schmidt6, Yiska Weisblum6, Bettina Salome Trüeb2,3, Inês Berenguer Veiga2,3, Kimberly Schmied2,3, Nadine Ebert2,3, Eleftherios Michailidis1, Avery Peace1, Francisco J Sánchez-Rivera7, Scott W Lowe7, Michael P Rout5, Theodora Hatziioannou6, Paul D Bieniasz6, John T Poirier8, Margaret R MacDonald1, Volker Thiel2,3, Charles M Rice1.
Abstract
Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.Entities:
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Year: 2021 PMID： 34648371 PMCID： PMC9007107 DOI： 10.1126/science.abj8430
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728