Methods to Quantify Cell Division and Hormone Gradients During Root Tropisms.

Tropisms are growth-based plant directional movements, allowing plants to respond to their living environments. Plant roots have developed various tropic responses, including gravitropism, hydrotropism, chemotropism, and halotropism, in response to the gravity, moisture gradient, nutrient gradient, and salinity gradient, respectively. Revealed mechanisms of several tropic responses suggested that plant hormone gradient and cell division activity play key roles in determining these responses. Approaches to measure cell division and hormone gradients, however, have rarely been applied in root tropic analyses. Here, we describe a number of methods to quantify cell division and hormone gradients during root tropic analysis. These approaches are mainly based on our previous researches on root hydrotropism.