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Literature DB >> 34643908

Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples.

Aline Rifflet^1,2, Caroline Demangel³, Laure Guenin-Macé⁴.

Abstract

Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.

Entities: Chemical

Keywords: Folch’s extraction; LC-MS/MS; Mycobacterium ulcerans; Mycolactone; TLC

Mesh：

Substances：

Year: 2022 PMID： 34643908 DOI： 10.1007/978-1-0716-1779-3_13

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

12 in total

Review 1. Sec61 blockade by mycolactone: A central mechanism in Buruli ulcer disease.

Authors: Caroline Demangel; Stephen High
Journal: Biol Cell Date: 2018-08-14 Impact factor: 4.458

Review 2. The chemistry and biology of mycolactones.

Authors: Matthias Gehringer; Karl-Heinz Altmann
Journal: Beilstein J Org Chem Date: 2017-08-11 Impact factor: 2.883

3. Heterogeneity of mycolactones produced by clinical isolates of Mycobacterium ulcerans: implications for virulence.

Authors: Armand Mve-Obiang; Richard E Lee; Françoise Portaels; P L C Small
Journal: Infect Immun Date: 2003-02 Impact factor: 3.441

4. On the origin of Mycobacterium ulcerans, the causative agent of Buruli ulcer.

Authors: Kenneth D Doig; Kathryn E Holt; Janet A M Fyfe; Caroline J Lavender; Miriam Eddyani; Françoise Portaels; Dorothy Yeboah-Manu; Gerd Pluschke; Torsten Seemann; Timothy P Stinear
Journal: BMC Genomics Date: 2012-06-19 Impact factor: 3.969

5. Mycolactone diffuses into the peripheral blood of Buruli ulcer patients--implications for diagnosis and disease monitoring.

Authors: Fred S Sarfo; Fabien Le Chevalier; N'Guetta Aka; Richard O Phillips; Yaw Amoako; Ivo G Boneca; Pascal Lenormand; Mireille Dosso; Mark Wansbrough-Jones; Romain Veyron-Churlet; Laure Guenin-Macé; Caroline Demangel
Journal: PLoS Negl Trop Dis Date: 2011-07-19

6. Mycolactone subverts immunity by selectively blocking the Sec61 translocon.

Authors: Ludivine Baron; Anja Onerva Paatero; Jean-David Morel; Francis Impens; Laure Guenin-Macé; Sarah Saint-Auret; Nicolas Blanchard; Rabea Dillmann; Fatoumata Niang; Sandra Pellegrini; Jack Taunton; Ville O Paavilainen; Caroline Demangel
Journal: J Exp Med Date: 2016-11-07 Impact factor: 14.307

Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples.

Review 1. Sec61 blockade by mycolactone: A central mechanism in Buruli ulcer disease.

Review 2. The chemistry and biology of mycolactones.

3. Heterogeneity of mycolactones produced by clinical isolates of Mycobacterium ulcerans: implications for virulence.

4. On the origin of Mycobacterium ulcerans, the causative agent of Buruli ulcer.

5. Mycolactone diffuses into the peripheral blood of Buruli ulcer patients--implications for diagnosis and disease monitoring.

6. Mycolactone subverts immunity by selectively blocking the Sec61 translocon.

7. Structure-activity relationship studies on the macrolide exotoxin mycolactone of Mycobacterium ulcerans.

8. A novel mycolactone toxin obtained by biosynthetic engineering.

Review 9. Mycolactones: immunosuppressive and cytotoxic polyketides produced by aquatic mycobacteria.

10. Kinetics of mycolactone in human subcutaneous tissue during antibiotic therapy for Mycobacterium ulcerans disease.

1. Mycobacterium ulcerans Experimental Dormancy.