Literature DB >> 34633842

Impact of Erg11 Amino Acid Substitutions Identified in Candida auris Clade III Isolates on Triazole Drug Susceptibility.

Benjamin Williamson1, Adam Wilk1, Kevin D Guerrero2, Timothy D Mikulski2, Tony N Elias2, Indira Sawh1, Geselle Cancino-Prado1, Dianne Gardam3, Christopher H Heath3,4,5,6, Nelesh P Govender7,8,9, David S Perlin2, Milena Kordalewska2, Kelley R Healey1.   

Abstract

ERG11 sequencing of 28 Candida auris clade III isolates revealed the presence of concomitant V125A and F126L substitutions. Heterologous expression of Erg11-V125A/F126L in Saccharomyces cerevisiae led to reduced fluconazole and voriconazole susceptibilities. Generation of single substitution gene variants through site-directed mutagenesis uncovered that F126L primarily contributes to the elevated triazole MICs. A similar yet diminished pattern of reduced susceptibility was observed with the long-tailed triazoles posaconazole and itraconazole for the V125A/F126L, F126L, Y132F, and K143R alleles.

Entities:  

Keywords:  African clade; Candida auris; ERG11; azole resistance; clade III; fluconazole resistance; heterologous expression; mutagenesis; short- and long-tailed triazoles

Mesh：

Substances：

Year:  2021        PMID： 34633842      PMCID： PMC8765314          DOI： 10.1128/AAC.01624-21

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.938


  27 in total

1.  A multicentre study of antifungal susceptibility patterns among 350 Candida auris isolates (2009-17) in India: role of the ERG11 and FKS1 genes in azole and echinocandin resistance.

Authors:  Anuradha Chowdhary; Anupam Prakash; Cheshta Sharma; Milena Kordalewska; Anil Kumar; Smita Sarma; Bansidhar Tarai; Ashutosh Singh; Gargi Upadhyaya; Shalini Upadhyay; Priyanka Yadav; Pradeep K Singh; Vikas Khillan; Neelam Sachdeva; David S Perlin; Jacques F Meis
Journal:  J Antimicrob Chemother       Date:  2018-04-01       Impact factor: 5.790

2.  Contribution of clinically derived mutations in ERG11 to azole resistance in Candida albicans.

Authors:  Stephanie A Flowers; Brendan Colón; Sarah G Whaley; Mary A Schuler; P David Rogers
Journal:  Antimicrob Agents Chemother       Date:  2014-11-10       Impact factor: 5.191

3.  Crystal Structures of Full-Length Lanosterol 14α-Demethylases of Prominent Fungal Pathogens Candida albicans and Candida glabrata Provide Tools for Antifungal Discovery.

Authors:  Mikhail V Keniya; Manya Sabherwal; Rajni K Wilson; Matthew A Woods; Alia A Sagatova; Joel D A Tyndall; Brian C Monk
Journal:  Antimicrob Agents Chemother       Date:  2018-10-24       Impact factor: 5.191

4.  Amino acid substitutions in the cytochrome P-450 lanosterol 14alpha-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents.

Authors:  D Sanglard; F Ischer; L Koymans; J Bille
Journal:  Antimicrob Agents Chemother       Date:  1998-02       Impact factor: 5.191

5.  Tracing the Evolutionary History and Global Expansion of Candida auris Using Population Genomic Analyses.

Authors:  Nancy A Chow; José F Muñoz; Anastasia P Litvintseva; Christina A Cuomo; Lalitha Gade; Elizabeth L Berkow; Xiao Li; Rory M Welsh; Kaitlin Forsberg; Shawn R Lockhart; Rodney Adam; Alexandre Alanio; Ana Alastruey-Izquierdo; Sahar Althawadi; Ana Belén Araúz; Ronen Ben-Ami; Amrita Bharat; Belinda Calvo; Marie Desnos-Ollivier; Patricia Escandón; Dianne Gardam; Revathi Gunturu; Christopher H Heath; Oliver Kurzai; Ronny Martin
Journal:  mBio       Date:  2020-04-28       Impact factor: 7.867

6.  Outbreak of Candida auris infection in a COVID-19 hospital in Mexico.

Authors:  Hiram Villanueva-Lozano; Rogelio de J Treviño-Rangel; Gloria M González; María Teresa Ramírez-Elizondo; Reynaldo Lara-Medrano; Mary Cruz Aleman-Bocanegra; Claudia E Guajardo-Lara; Natalia Gaona-Chávez; Fernando Castilleja-Leal; Guillermo Torre-Amione; Michel F Martínez-Reséndez
Journal:  Clin Microbiol Infect       Date:  2021-01-08       Impact factor: 8.067

7.  Positive correlation between Candida auris skin-colonization burden and environmental contamination at a ventilator-capable skilled nursing facility in Chicago.

Authors:  D Joseph Sexton; Meghan L Bentz; Rory M Welsh; Gordana Derado; William Furin; Laura J Rose; Judith Noble-Wang; Massimo Pacilli; Tristan D McPherson; Stephanie Black; Sarah K Kemble; Owen Herzegh; Ausaf Ahmad; Kaitlin Forsberg; Brendan Jackson; Anastasia P Litvintseva
Journal:  Clin Infect Dis       Date:  2021-05-12       Impact factor: 9.079

8.  Triazole resistance mediated by mutations of a conserved active site tyrosine in fungal lanosterol 14α-demethylase.

Authors:  Alia A Sagatova; Mikhail V Keniya; Rajni K Wilson; Manya Sabherwal; Joel D A Tyndall; Brian C Monk
Journal:  Sci Rep       Date:  2016-05-18       Impact factor: 4.379

9.  Limited ERG11 Mutations Identified in Isolates of Candida auris Directly Contribute to Reduced Azole Susceptibility.

Authors:  Kelley R Healey; Milena Kordalewska; Cristina Jiménez Ortigosa; Ashutosh Singh; Indira Berrío; Anuradha Chowdhary; David S Perlin
Journal:  Antimicrob Agents Chemother       Date:  2018-09-24       Impact factor: 5.191
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  1 in total

1.  Transcriptional Response of Candida auris to the Mrr1 Inducers Methylglyoxal and Benomyl.

Authors:  Amy R Biermann; Deborah A Hogan
Journal:  mSphere       Date:  2022-04-27       Impact factor: 5.029
  1 in total

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