| Literature DB >> 34628012 |
Rosy Sultana1, Taufiqur Rahman Bhuiyan2, Afsana Shirin Sathi2, Salma Sharmin2, Sharmina Yeasmin3, Muhammad Ikhtear Uddin2, Md Saruar Bhuiyan2, Kaiissar Mannoor4, Muhammad Manjurul Karim5, K Zaman2, Firdausi Qadri6.
Abstract
Serological analysis is an integral part of laboratory practice nowadays. The present study was aimed to develop and validate a modified Enzyme linked Immunosorbent Assay (ELISA) for determination of IgG antibody against Hepatitis E Virus (HEV) using dried blood spots (DBS) and corresponding plasma samples. A total of 65 samples (45 HEV patients, 20 healthy controls) were analyzed. DBS and plasma samples demonstrated equivalent optical densities for detecting anti-HEV IgG. A highly significant correlation was observed between plasma and DBS sample absorbances (R2 = 0.98; p < 0.001) at dilution 1:200, indicating true agreement between the two procedures. The assay exhibited decent linearity and showed no effect of physiological hematocrit on assay performance. Data suggested recommendable promise in using DBS as a suitable alternative to plasma samples to determine HEV IgG antibody evidenced by significant correlation with plasma results. Therefore, identical method for processing DBS specimens including it's proper storage is recommended for implementation of a modified ELISA in different settings.Entities:
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Year: 2021 PMID： 34628012 PMCID： PMC8960178 DOI： 10.1016/j.micinf.2021.104890
Source DB: PubMed Journal: Microbes Infect ISSN： 1286-4579 Impact factor: 2.700