Literature DB >> 34626394

Advances in Studying Glycosaminoglycan-Protein Interactions Using Capillary Electrophoresis.

Aiye Liang1, Umesh Desai2.   

Abstract

Methods for studying interactions between glycosaminoglycans (GAGs) and proteins have assumed considerable significance as their biological importance increases. Capillary electrophoresis (CE) is a powerful method to study these interactions due to its speed, high efficiency, and low sample/reagent consumption. In addition, CE works effectively under a wide range of physiologically relevant conditions. This chapter presents the state of the art on CE methods for studying GAG-protein interactions including affinity capillary electrophoresis (ACE), capillary zone electrophoresis (CZE), frontal analysis (FA)/frontal analysis continuous capillary electrophoresis (FACCE), and capillary electrokinetic chromatography (CEC) with detailed experimental protocols for ACE and CZE methods.
© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

Entities:  

Keywords:  Affinity capillary electrophoresis; Biophysical technique; Capillary electrophoresis; Capillary zone electrophoresis; GAG–protein interactions; Glycosaminoglycans; Heparin

Mesh：

Substances：

Year:  2022        PMID： 34626394     DOI： 10.1007/978-1-0716-1398-6_30

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  65 in total

1.  Microscale characterization of the structure-activity relationship of a heparin-binding glycopeptide using affinity capillary electrophoresis and immobilized enzymes.

Authors:  N H Heegaard
Journal:  J Chromatogr A       Date:  1999-08-20       Impact factor: 4.759

Review 2.  Capillary electrophoresis for the study of affinity interactions.

Authors:  N H Heegaard
Journal:  J Mol Recognit       Date:  1998       Impact factor: 2.137

Review 3.  Order out of complexity--protein structures that interact with heparin.

Authors:  B Mulloy; R J Linhardt
Journal:  Curr Opin Struct Biol       Date:  2001-10       Impact factor: 6.809

Review 4.  Interactions of charged ligands with beta(2)-microglobulin conformers in affinity capillary electrophoresis.

Authors:  Niels H H Heegaard; Ersilia De Lorenzi
Journal:  Biochim Biophys Acta       Date:  2005-08-19

Review 5.  Heparan sulfate: a complex polymer charged with biological activity.

Authors:  John M Whitelock; Renato V Iozzo
Journal:  Chem Rev       Date:  2005-07       Impact factor: 60.622

6.  Demonstration of a heparin-binding site in serum amyloid P component using affinity capillary electrophoresis as an adjunct technique.

Authors:  N H Heegaard; H D Mortensen; P Roepstorff
Journal:  J Chromatogr A       Date:  1995-11-24       Impact factor: 4.759

7.  Interaction of the N-terminal domain of apolipoprotein E4 with heparin.

Authors:  J Dong; C A Peters-Libeu; K H Weisgraber; B W Segelke; B Rupp; I Capila; M J Hernáiz; L A LeBrun; R J Linhardt
Journal:  Biochemistry       Date:  2001-03-06       Impact factor: 3.162

8.  Structural studies of octasaccharides derived from the low-sulfated repeating disaccharide region and octasaccharide serines derived from the protein linkage region of porcine intestinal heparin.

Authors:  S Yamada; K Sakamoto; H Tsuda; K Yoshida; M Sugiura; K Sugahara
Journal:  Biochemistry       Date:  1999-01-12       Impact factor: 3.162

9.  Investigation on the binding site in heparin by spectrophotometry.

Authors:  Q C Jiao; Q Liu; C Sun; H He
Journal:  Talanta       Date:  1999-05       Impact factor: 6.057

10.  Differences in the interaction of heparin with arginine and lysine and the importance of these basic amino acids in the binding of heparin to acidic fibroblast growth factor.

Authors:  J R Fromm; R E Hileman; E E Caldwell; J M Weiler; R J Linhardt
Journal:  Arch Biochem Biophys       Date:  1995-11-10       Impact factor: 4.013
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