Induction of Constitutive Androstane Receptor during the Development of Oxidative Stress.

We studied the effect of 3-, 24-, and 72-h exposure to H2O2 in concentrations of 0.1-100.0 μM on the level of constitutive androstane receptor in Caco-2 cells. It was shown that 3- and 24-h incubation with Н2О2 in all concentrations had no effect on the level of constitutive androstane receptors. Increasing the incubation time to 72 h led to an increase in the level of constitutive androstane receptor at H2O2 concentrations of 5, 10, and 50 μM and to a decrease at a concentration of 100 μM. Antioxidant glutathione (1 mM) in parallel to the prooxidant neutralized these changes.