Foroogh Makki1, Zahra Boroomand2, Mansour Mayahi3, Masoud Reza Seyfi Abad Shapouri4. 1. Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, Iran. 2. Department of Clinical Sciences, Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, Iran. z.boroomand@scu.ac.ir. 3. Department of Clinical Sciences, Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, Iran. 4. Department of Pathobiology, Faculty of Veterinary Medicine, ShahidChamran University of Ahvaz, Ahvaz, Iran.
Abstract
BACKGROUND: Newcastle disease, is one of the most important diseases of the poultry industry, has many economic losses. The aim of this study was to isolate and determine the molecular identity of Newcastle disease virus in 40 broiler flocks with respiratory symptoms in four provinces of Iran. METHODS AND RESULTS: Samples of farms with respiratory symptoms were collected from different regions of Isfahan, East Azerbaijan, Golestan, and Khuzestan provinces and inoculated into 9-day-old embryonated chicken eggs. The Reverse-transcription polymerase chain reaction (RT-PCR) was performed to detect the Newcastle disease virus in allantoic fluid. Of the 40 flocks, the virus was isolated and identified in 16 flocks. The PCR products of 16 isolates were sequenced, and a phylogenetic tree was drawn. Accordingly, six isolates were in genotype II and ten isolates were in subgenotype VII.1.1 (VIId) of class II. CONCLUSION: Both genotypes were present in all four provinces. The isolates of Khuzestan province showed the greatest diversity compared to the other three provinces. The similarity of isolates belonging to genotype II in this study was observed with Pakistan, China, and Nigeria, and other isolates were similar to previous isolates in Iran. Also, the highest amino acid sequence in the F-protein cleavage site was 112RRQKR/F117 for VII.1.1 (VIId) genotype isolates and 112GRQGR/L117 for II genotype isolates.
BACKGROUND: Newcastle disease, is one of the most important diseases of the poultry industry, has many economic losses. The aim of this study was to isolate and determine the molecular identity of Newcastle disease virus in 40 broiler flocks with respiratory symptoms in four provinces of Iran. METHODS AND RESULTS: Samples of farms with respiratory symptoms were collected from different regions of Isfahan, East Azerbaijan, Golestan, and Khuzestan provinces and inoculated into 9-day-old embryonated chicken eggs. The Reverse-transcription polymerase chain reaction (RT-PCR) was performed to detect the Newcastle disease virus in allantoic fluid. Of the 40 flocks, the virus was isolated and identified in 16 flocks. The PCR products of 16 isolates were sequenced, and a phylogenetic tree was drawn. Accordingly, six isolates were in genotype II and ten isolates were in subgenotype VII.1.1 (VIId) of class II. CONCLUSION: Both genotypes were present in all four provinces. The isolates of Khuzestan province showed the greatest diversity compared to the other three provinces. The similarity of isolates belonging to genotype II in this study was observed with Pakistan, China, and Nigeria, and other isolates were similar to previous isolates in Iran. Also, the highest amino acid sequence in the F-protein cleavage site was 112RRQKR/F117 for VII.1.1 (VIId) genotype isolates and 112GRQGR/L117 for II genotype isolates.
Authors: Chantal J Snoeck; Ademola A Owoade; Emmanuel Couacy-Hymann; Bello R Alkali; Mbah P Okwen; Adeniyi T Adeyanju; Giscard F Komoyo; Emmanuel Nakouné; Alain Le Faou; Claude P Muller Journal: J Clin Microbiol Date: 2013-05-08 Impact factor: 5.948
Authors: Mohammad Javad Mehrabanpour; Setareh Khoobyar; Abdollah Rahimian; Mohammad Bagher Nazari; Mohammad Reza Keshtkar Journal: Vet Res Forum Date: 2014 Impact factor: 1.054