| Literature DB >> 34595727 |
Antônio Augusto Fonseca Júnior1,2, Luciana Rabello Ferreira3, Mateus Laguardia-Nascimento3, Anselmo Vasconcelos Rivetti Júnior3, Bruna Lopes Bueno4, Jenner Karlisson Pimenta Dos Reis4, Marcelo Fernandes Camargos3.
Abstract
Bovine leukemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus. BLV infects cattle worldwide and is responsible for significant economic losses. The objective of this study was to validate real-time quantitative PCR (qPCR) for the detection of BLV. After identification of the most efficient qPCR, the limits of detection, repeatability, and reproducibility were determined. The results indicate that qPCR can be easily reproduced between laboratories with high sensitivity. The test variation was low in samples from lesions suggestive of bovine leukosis or whole blood.Entities:
Keywords: Bovine leukemia virus; Real-time PCR; Validation
Mesh：
Year: 2021 PMID： 34595727 PMCID： PMC8578338 DOI： 10.1007/s42770-021-00613-0
Source DB: PubMed Journal: Braz J Microbiol ISSN： 1517-8382 Impact factor: 2.214