Literature DB >> 34595323

Genotyping-free Selection of Double Allelic Gene Edited Medaka Using Two Different Fluorescent Proteins.

Yu Murakami1, Satoshi Ansai1,2, Akari Yonemura1, Masato Kinoshita1.   

Abstract

This protocol describes a simple genotyping using two different colors of fluorescent protein genes inserted at the target locus. This method makes it possible to determine the genotype of each individual simply by observing the fluorescence later than F1 generation.
Copyright © 2017 The Authors; exclusive licensee Bio-protocol LLC.

Entities:  

Keywords:  CRISPR/Cas; Fluorescent reporter gene; Gene knock-in; Genome editing; Medaka

Year:  2017        PMID： 34595323      PMCID： PMC8438436          DOI： 10.21769/BioProtoc.2665

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  3 in total

1.  Characterization of a nervous system-specific promoter for growth-associated protein 43 gene in Medaka (Oryzias latipes).

Authors:  Kazuhiro E Fujimori; Takashi Kawasaki; Tomonori Deguchi; Syunsuke Yuba
Journal:  Brain Res       Date:  2008-10-07       Impact factor: 3.252

2.  An efficient system for homology-dependent targeted gene integration in medaka (Oryzias latipes).

Authors:  Yu Murakami; Satoshi Ansai; Akari Yonemura; Masato Kinoshita
Journal:  Zoological Lett       Date:  2017-07-06       Impact factor: 2.836

3.  CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo.

Authors:  Miguel A Moreno-Mateos; Charles E Vejnar; Jean-Denis Beaudoin; Juan P Fernandez; Emily K Mis; Mustafa K Khokha; Antonio J Giraldez
Journal:  Nat Methods       Date:  2015-08-31       Impact factor: 28.547
  3 in total

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